What's going on with this in vitro seedling?

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MC

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May 22, 2017, 11:50:29 AM5/22/17
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Here's a picture of an Epidendrum seedling growing in vitro. What is the blob structure? Is it a callus, vitrification, or something else? I've noticed similar growths on some of my other cultures as well. Thanks.
epidendrum.jpg

Rikke Rasmussen

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May 23, 2017, 2:04:05 PM5/23/17
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Looks like callus.

What kind of media are you growing on? And are they seed-started cultures?

On Mon, May 22, 2017 at 8:50 AM, MC <marky...@gmail.com> wrote:
Here's a picture of an Epidendrum seedling growing in vitro. What is the blob structure? Is it a callus, vitrification, or something else? I've noticed similar growths on some of my other cultures as well. Thanks.

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Jeff Backstrom

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May 26, 2017, 1:47:25 AM5/26/17
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Callus. Sometimes they grow out of it, sometimes they continue to multiply, particularly if the medium is too rich in sugars.

On Mon, May 22, 2017 at 8:50 AM, MC <marky...@gmail.com> wrote:
Here's a picture of an Epidendrum seedling growing in vitro. What is the blob structure? Is it a callus, vitrification, or something else? I've noticed similar growths on some of my other cultures as well. Thanks.

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