You were using an electroporation cuvette, right? (I can't tell)
Here is a protocol that worked well for me, with E.coli overnight
subcultures and 18MOhm water... your medium may have been too salty
(conductive):
https://groups.google.com/forum/#!msg/diybio/JcKyNIQGLIM/u4qIOllQRnAJ
Also, FYI:
Here are tables of voltage settings for various organisms...
http://m.imgur.com/a/swOXq
Also from this BioRad doc, pg 28
http://www.bio-rad.com/webroot/web/pdf/lsr/literature/4006174B.pdf
(screenshot here too)
http://imgur.com/TmwPOho
Most Interestingly: E.Coli 12-18kV/cm, test at 5msec pulse, adjust
from that starting point for efficiency tuning
S.cerevisieae ~7.5kV/cm, start at 5msec pulse
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