ReSeq crowd funder to write new software for HiSeq DNA sequencers

[Kaspar Emanuel]

Hey folks,

Hope this isn't against your the rules or etiquette of your group. A commenter on our campaign suggested I post this here. We want to write free and open source software so we people re-use the HiSeq 2000 and 2500 DNA sequencers and have a modest crowd-funder going.

https://wemakeit.com/projects/reseq-reuse-dna-sequencers

These machines cost over half a million dollars not too long ago but now you can get them very cheaply and it would be a shame to let them go to waste. We still need quite a bit of support to make it happen.

Cheers,

Kaspar

[Marc Juul]

Hi! I was the one to suggest you post here. What an inspiring project!

These are extremely powerful tools and making them accessible to the
average biohackerspace would be incredibly valuable!

Repurposing high technology and putting it into the hands of the
people. This is the very essence of hacking!

On Wed, Apr 24, 2019 at 7:07 PM Marc Juul <ma...@juul.io> wrote:

Hi! I was the one to suggest you post here. What an inspiring project!

These are extremely powerful tools and making them accessible to the
average biohackerspace would be incredibly valuable!

Repurposing high technology and putting it into the hands of the
people. This is the very essence of hacking!

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[Abizar Lakdawalla]

Hi folks, any plan on getting the consumables for the HiSeq? They cost US$10ks per run if they are bought from Illumina.

Abizar

To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/CAL4ejvT_gph2kNdb2uLAN4fzQOedzdJUQ-Fc2QM6wWY_a3-_9A%40mail.gmail.com.

[John Griessen]

On 4/24/19 10:23 PM, Abizar Lakdawalla wrote:
> any plan on getting the consumables for the HiSeq? They cost US$10ks per run if they are bought from Illumina.

My take on reading the https://wemakeit.com/projects/reseq-reuse-dna-sequencers pages is that
components will be developed for uses first and maybe some day allow open uses, but not really in conflict with Illumina's
patents, and so not using their consumables. They did not say they were going to make the machines run as they did
when new.

Driving them as an automated microscope was first on the to do list.

[Kaspar Emanuel]

Hi folks,

John is right, the campaign is only for writing software to allow people to drive the machines. You will be able to use the Micromanager interface and scripting capabilities to do whatever you want, including patented procedures, if you can get your hands on the consumables and if you aren't exploiting them commercially, but IANAL, just a software developer.

Looks like this will be a close call as to whether we will reach our funding goal, any suggestions on where else to post are very much appreciated.

Cheers,

Kaspar

[Dakota Hamill]

This guy is cool.  A lot of people I know in sequencing mention his blog.  (I know next to nothing about NGS).  Maybe he'd give you a shoutout, maybe not. 

http://omicsomics.blogspot.com/

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[Abizar Lakdawalla]

Will Greenleaf at Stanford had hacked Illumina sequencers as imaging platforms. Might be worth contacting him.

To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/CAGdeWmSOArDYk3g8GLPHSx%2BGMCGPT3JZqbAk8yCsy_hbDu2crQ%40mail.gmail.com.

[Kaspar Emanuel]

Thanks for the tips folks. I reached out to your suggestions. We are not tantalizingly close to making it!

On Saturday, April 27, 2019 at 4:33:57 PM UTC, Abizar Lakdawalla wrote:

Will Greenleaf at Stanford had hacked Illumina sequencers as imaging platforms. Might be worth contacting him.

On Sat, Apr 27, 2019 at 9:07 AM Dakota Hamill <dko...@gmail.com> wrote:

This guy is cool.  A lot of people I know in sequencing mention his blog.  (I know next to nothing about NGS).  Maybe he'd give you a shoutout, maybe not. 

http://omicsomics.blogspot.com/

On Sat, Apr 27, 2019 at 11:57 AM Kaspar Emanuel <kaspar...@gmail.com> wrote:

Hi folks,

John is right, the campaign is only for writing software to allow people to drive the machines. You will be able to use the Micromanager interface and scripting capabilities to do whatever you want, including patented procedures, if you can get your hands on the consumables and if you aren't exploiting them commercially, but IANAL, just a software developer.

Looks like this will be a close call as to whether we will reach our funding goal, any suggestions on where else to post are very much appreciated.

Cheers,

Kaspar

On Thursday, April 25, 2019 at 3:05:18 PM UTC, John Griessen wrote:

On 4/24/19 10:23 PM, Abizar Lakdawalla wrote:
> any plan on getting the consumables for the HiSeq? They cost US$10ks per run if they are bought from Illumina.

My take on reading the https://wemakeit.com/projects/reseq-reuse-dna-sequencers pages is that
components will be developed for uses first and maybe some day allow open uses, but not really in conflict with Illumina's
patents, and so not using their consumables.  They did not say they were going to make the machines run as they did
when new.

Driving them as an automated microscope was first on the to do list.

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