Challenges building a DNA synthesizers?

[Open BioLab Graz - Austria]

Hiho,

Does anyone here maybe know what the challenges in building a dna synthesizer are?

I havent really got insight into this specific technique, but it seems as if these machines just pump a series of liquids..

[J Adams]

A synthesizer by it’s very nature is an automated fluid delivery devise that delivers fluids in a specific sequence with specific timing to provide automated DNA and RNA synthesis.  The process that the machines ALL run are Deprotection (i.e. remove the 5’ Trityl group), coupling (extend 1 base, with activator), capping (an acetylation reaction requiring acetic anhydride and NMI) and oxidation (convert the P3 to a stable P5), then they repeat this cycle over and over again for the full length of the oligonucleotide being synthesized.

 

So, ALL SYNTHESIZERS ARE THE SAME.  There are slight differences, but, all are fluid delivery modules that deliver fluid in an automated fashion to perform automated DNA and RNA synthesis.

 

I hope this helps.

 

Regards;

 

 

 

 

J Adams

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[Nathan McCorkle]

Some of the really cool ideas in research are using nanochannels to
isolate diffusion to as little of the growing end of the DNA chain,
but otherwise the wikipedia article on Oligonucleotide synthesis
covers what J Adams said plus a lot of the history.
http://en.wikipedia.org/wiki/Oligonucleotide_synthesis

> https://groups.google.com/d/msgid/diybio/03d801ce566b%2471256a90%2453703fb0%24%40com?hl=en.

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-Nathan

[Open BioLab Graz - Austria]

Dealing with multible fluids shouldnt be a big problem, but I wonder where the synthesis itself happens. What does the 'reaction chamber' look like? As far as i know you have to fix the starting molecules of the dna somehow?