Homemade DIYBio: LB medium

[Willian Barela Costa]

As everybody knows LB medium is the most common liquid media for culturing bacteria. For a biohacker is important to have in garage this medium for severeal experiences. 

Actually I have bioluminescent bacterias E.coli modified and I'm tring to think how produce LB medium at low cost.

Let's try hack - Formula: 

10g Trytone

5 g yeast extract

10g NaCl

Yeast extract and NaCl is cheap at the market, but the Trytone isn't. Thinking how produce trytone, basically is the use of a protease the trypsin in the casein. The casein is easy to obtein from milk, but the trypsin is the problem. Trypsin is usually found in digestive system of many vertebrates, for some sellers, they produce in E.Coli mofied and anothers extracted from herb (I don't know which herbs). Other form to approach this problem is to replace Trytone for other ingredient, I think to use products like Whey protein or use the own casein and pray to bacteria degrade.

Someone could help me to think in another solution or insight ?

ty for everybody pay attetion in this problem

[Cathal Garvey]

I've used soy protein isolate to good effect. If you want, add bromelain (enzyme found in health stores) to "trypsinise" it.

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Sent from my Android device with K-9 Mail. Please excuse my brevity.

[Abizar Lakdawalla]

Protein supplements products used by bodybuilders might work. They are hydrolysates of casein, etc.

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[Patrik D'haeseleer]

How difficult is it to actually order LB powder over eBay or Amazon in your country?

One of the hits I see is $34.71 for half a kilogram, which is enough to make 20L of LB broth, or $1.74 per liter.

I know it's fun to plan for how to grow E. coli after the zombie apocalypse when all human civilization (or at least eBay and Amazon) has broken down. But for any real-world application, I would look towards buying dirt cheap and easily available commercial LB powder first.

I know microbiological supplies are much more strictly regulated in other countries though. Just wondering how widespread a phenomenon that is. And how many of those countries regulate LB powder, but not yeast extract? 

Patrik

[Kermit Henson]

I think  Patrick is right. Is much easier to get LB agar from ebay or similar resources. 

In case you want a homemade version, Cathal is right with the bromelain enzyme.

In my experience, using supplements products for bodybuilders can give you some problem. Once I tried a brand that made a big mess when I autoclaved it.

[Otto Heringer]

Hi William, how you doing?

I found this place where you can buy it (I bought stuff from them myself some time ago), but it is 3 times more expensive per weight than Patrik suggested.

Bodybuilder's products sounds like something fun to try.

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[Pieter Waag]

Also Simon Park's guide is probably still one of the best manuals for kitchen microbiology out there:
https://exploringtheinvisible.com/2012/11/05/94/

[Josiah Zayner]

Though some bacteria and yeast have specific media requirements many bacteria and yeast only need sugar and a nitrogen source. Some bacteria like E. coli also like salt and it is a good selective media but you can literally put sugar and some weight lifter protein, ammonium chloride which is supppper cheap(use tap water not distilled as tap water has other good stuff Ca, Mg, &c) and the bacteria or yeast will grow.

As Patrik said LB is cheaappp. The ODIN sells 100g for $12(https://www.the-odin.com/lb-powdered-media/)! Enough for 5L, which should last a biohacker a fair bit of time, especially if you are growing stuff in 10mL - 50mL increments.

Josiah Zayner