Ghost Heart: video

[Matthew Harbowy]

http://www.youtube.com/watch?v=ZrKcu9ua95U

counterculturelabs.org

biocurious.org

[Patrik D'haeseleer]

Great job on the video, Matt! Love the music.

For those of you who haven't been following the Ghost Heart saga: we decellularized a pig heart as a Halloween project, stripping out all the cells by perfusing the heart muscle with trypsin, detergent, bile acid, and oxyclean/ethanol - and lots of water and high saline rounds in between.

The purpose of decellularization an organ is to then reseed the remaining scaffold of connective tissue with a patient's own stem cells, regenerating an intact organ that can then be implanted into the patient without any tissue rejection issues. (see Tissue engineering: How to build a heart). The last part - reseeding with stem cells to regenerate an organ ready for transplantation - is still years of research away from clinical use. We just wanted to have a Ghost Heart in a jar, as a show piece!

You can find the official protocol here:

Preparation of Cardiac Extracellular Matrix from an Intact Porcine Heart

And here's a wonderful video on Jove showing the procedure:

Procedure for decellularization of porcine heart by retrograde coronary perfusion.

We took some shortcuts along the way, but for a show piece it came out wonderful. We'll be writing up exactly what we did soon. Meanwhile, check out a selection of the best pics in our Meetup gallery:

http://www.meetup.com/Counter-Culture-Labs/photos/18280182/

[Mega [Andreas Stuermer]]

Hehe. Guess you could sell those :D

[Mega [Andreas Stuermer]]

Btw, build in a motir device that makes it seem to beat. Using induction/wireless energy transmission. ;)

[Mega [Andreas Stuermer]]

Motor could come from an old cellphone, though that would vibrate instead of "beating"

[Nathan McCorkle]

I was actually thinking you might be able to make the heart 'beat' by
pulsing the circulation pumps in the right sequence... one hooked up
to the blood side, the other the lung side

On Mon, Nov 4, 2013 at 3:24 AM, Mega [Andreas Stuermer]
<masters...@gmail.com> wrote:
> Btw, build in a motir device that makes it seem to beat. Using induction/wireless energy transmission. ;)
>

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--
-Nathan

[Dan]

[Patrik D'haeseleer]

On Monday, November 4, 2013 3:23:14 AM UTC-8, Mega [Andreas Stuermer] wrote:

Hehe. Guess you could sell those :D

Yeah, I was just doing the math on that. We could offer them as a reward on our upcoming kickstarter for Counter Culture Labs, but they'll be a bit pricy. We should be able to get the cost down to ~$150 ($100 if we leave out the deoxycholic acid), plus a full day's labor. Rule of thumb is that you spend about 1/3 of your kickstarter revenue on rewards, so that would put it around the $300-500 reward level.

Patrik

[Patrik D'haeseleer]

On Monday, November 4, 2013 9:06:55 AM UTC-8, Nathan McCorkle wrote:

I was actually thinking you might be able to make the heart 'beat' by
pulsing the circulation pumps in the right sequence... one hooked up
to the blood side, the other the lung side

I considered inserted a little hand-pumped balloon for the Halloween show at the California Academy of Sciences. The Ghost Heart is quite floppy, so it shouldn't take much force to achieve a fairly realistic looking pumping movement.

Patrik

[Nathan McCorkle]

On Mon, Nov 4, 2013 at 10:21 AM, Dan <djwr...@gmail.com> wrote:

Thanks for the video Dan!

Just saw this, for keeping hearts alive between transports:
http://www.upworthy.com/it-looks-like-something-you-should-never-see-meet-a-medical-advancement-that-is-hard-to-forget-5

[adamg]

Hi Patrick,

Is there anyway conduct a perfusion decellurization at home?
I'm attempting to reduce some shop bought organs to a structural scaffold for a project yet have no lab space or equipment.
I am not looking to achieve a particularly high level of precision, but would love any suggestions you may have.

Are there any household products containing trypsin?