Radio-isotope and bacteria

[Busuttil]

Hello everyone,

 

What are the processes involved to attach a radio-isotope to bacteria?

 

Kevin

[Matthew Pocock]

Hi Kevin,

The easiest way to radio label bacteria is to feed them on a radio labeled carbon source. They will take it up and incorporate the isotope into its body.

Working with radioactivity would not be my first choice. You have to be very careful how you dispose of everything and wear extra protection and other safety precautions. Can I ask what you are wishing to do? There may be a way to do it without radio labeling.

Matthew

Sent from my android - may contain predictive text entertainment.

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[Erico Perrella]

What is the general idea (the one you want to do)?

I think maybe using some biosorption techniques could help you. There are lots of guys using biosorption around the world, and in my lab we do it mainly using radioactive metals.

http://biosorption.mcgill.ca/publication/PDFs/101-BP'95-11,235-50-RevHolan.pdf

you can also take a look at this: FERREIRA, Rafael Vicente de Padua ; Sakata, Solange Kazumi ; Dutra, Fernando ; Vitta, Patricia Busko ; Taddei, Maria Helena Tirollo ; BELLINI, Maria Helena ; Marumo, Júlio Takehiro . Treatment of radioactive liquid organic waste using bacteria community. Journal of Radioanalytical and Nuclear Chemistry (Print), v. 1, p. 1, 2011.

If you want it I can get you a copy.

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[Busuttil]

Hi Erico,

 

Thanks for your reply. The idea behind it is to have a radio-isotope such as Rhenium-188 attached to a varicella strain (which is a virus and not a bacteria as I erroneously mentioned in the original question), which in turn is injected intravenously into a human being in order to eradicate malignant cancer cells particularly when the disease has already metastatised.

 

Thanks

 

Kevin

[Busuttil]

Hi Matthew,

 

The main purpose is to use a radio-isotope, more specifically Rhenium-188, attached to a chicken pox strain to treat my wife for pancreatic cancer. My wife, 32 year old, was diagnosed with metastatic pancreatic cancer after giving birth to our only child. How difficult is it to find a lab that supplies an attentuated chicken pox virus with Rhenium-188?

 

Thanks

 

Kevin

[Cathal Garvey]

I don't disagree, best avoid radioisotopes where other options present
themselves. However, there is too much hype about radiation and
radioisotopes, so depending on what you're working with it may not be
an issue. i.e., what's the "Banana Equivalent Dose" of the radioisotope
type/age/quantity you're using? :)

Still, particularly for those isotopes that could end up in your DNA,
may be a decent rule of thumb to avoid them and stick to dyes or
fluorophores.

[Nathan McCorkle]

Hmm, very neat idea. There is plenty of data on conjugating Rhenium to
proteins, so you might look into the virion surface proteins and
google scholar search that protein name with rhenium.

keywords: labelling radio-labelling radio conjugate phage virion

here are some papers I came up with just now

Labeling Biomolecules with Radiorhenium - a Review of the Bifunctional Chelators
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1949414/

http://diyhpl.us/~nmz787/pdf/A_Novel_Fluorescent_Probe_Europium_Complex_Hybridized_T7_Phage.pdf

http://en.wikipedia.org/wiki/Radioisotope_renography

http://www.freepatentsonline.com/y2013/0116408.html

http://f3.tiera.ru/2/B_Biology/BH_Human/Reilly%20R.M.%20(ed.)%20Monoclonal%20Antibody%20and%20Peptide-Targeted%20Radiotherapy%20of%20Cancer%20(Wiley,%202010)(ISBN%200470243724)(O)(659s)_BH_.pdf#page=128

http://www.google.com/patents?hl=en&lr=&vid=USPAT6706252&id=OQwRAAAAEBAJ&oi=fnd&dq=labelling+Rhenium+virus+envelope&printsec=abstract#v=onepage&q&f=false



I've used the protein linking kit at the end of this page before (with
plastic microspheres), it was quite simple:
http://www.bangslabs.com/products/accessory_reagents

That's assuming there are some free COOHs to bond with, they also sell
other kits for different chemistry. My point is, the linking chemistry
need not be too difficult in practice. Finding someone to try a
treatment that is novel/extraordinary is another thing.

Best luck, what you want is certainly possible, but you're gonna need
to find some specialist doctor who trusts molecular biologists and
maybe a chemist. Most MDs wouldn't know the carboxy terminus from the
amino of a protein... finding one that knows that much might be a
start.

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--
-Nathan