Using Crispr on plants

103 views
Skip to first unread message

Anonymous Anonymous

unread,
Oct 26, 2016, 8:03:47 PM10/26/16
to DIYbio


Hello, I'm interested in getting started in DIYbio, I live in Miami where there doesn't really seem to be a community for DIYbio.
I think I could scrape some money together for basic lab equipment, but my main question is, what is the primary procedure for using Crispr in plants?
From the articles I've read, it seems that the only eukaryotic organism that's easily "Crispr'd" is yeast, although labs seem to be moving to plants.
Is using Crispr to gene-edit plants within the reach of an amateur?

Sebastian S Cocioba

unread,
Oct 26, 2016, 10:38:29 PM10/26/16
to diy...@googlegroups.com
I'd strongly recommend practicing with agrobacterium since its reliable albeit random insertions. Its easier to work with and has a lot less theory to understand than CRISPR. Ask around local university plant departments!

Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diy...@googlegroups.com. To unsubscribe from this group, send email to diybio+un...@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+un...@googlegroups.com.
To post to this group, send email to diy...@googlegroups.com.
Visit this group at https://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/69986175-d606-4a27-a817-b613eb9cddc6%40googlegroups.com.
For more options, visit https://groups.google.com/d/optout.

Koeng

unread,
Oct 26, 2016, 11:22:53 PM10/26/16
to DIYbio
It's important to note the only reason CRISPRing yeast is easy is that they are easy to transform (I use CRISPR in yeast occasionally for point mutation selection). 

The entire problem is transformation. Do what Sebastian says, Agro is the best way to transform plants (for DIYers, in my opinion)

-Koeng

Tharindu Sampath

unread,
Jul 3, 2017, 7:32:58 PM7/3/17
to DIYbio

Best Plants In Sri Lanka

Visit Us- http://www.polwattaplantnursery.com

Tharindu Sampath

unread,
Jul 3, 2017, 7:33:20 PM7/3/17
to DIYbio

Best Plants In Sri Lanka

Visit Us- http://www.polwattaplantnursery.com


On Thursday, October 27, 2016 at 5:33:47 AM UTC+5:30, Anonymous Anonymous wrote:
Reply all
Reply to author
Forward
0 new messages