Request for Artificial gene synthesis

[Shambo Hore]

Dear all.
Please refer any DIY method for artificial genetic code synthesis. If there any ongoing project regarding with this matter, i will be glad to participate with it.

Thank you.
Shonku.

[CodeWarrior]

afaik no one is doing it but ideas do get thrown around https://groups.google.com/forum/#!topic/diybio/FXaT-MjJQYI

[Nathan McCorkle]

I have been working on trying to make a complete easy front-to-back
system for microfluidic product, with the intent on DNA synthesis.
Recently I bought this commercial blu-ray laser (XBox of some sort
replacement) that these folks reverse-engineered all the controls for,
and then built a circuit-board to enable all functionality and control
for anyone:
http://www.diyouware.com/DiyoPCB-MKI

This will achieve sub-micron spot and feature sizes, enabling
microfluidic circuitry to be written in photoresist. I have, for now,
purchased a used spin-coater, but I did previously find a vacuum
bearing for about $11 which would enable a spin-coater to be built
with a BLDC motor and simple vacuum pump pretty easily. I have been
focusing more on the CAD and CAM things lately (I have local changes
that I have been meaning to push, such as a 3-finger peristaltic pump
model):
https://github.com/nmz787/python-brlcad-tcl/tree/master/python-brlcad-tcl

some kicad models (electrical not fluidic) converted to g-code,
direct-laser exposed photoresist which I spincoated:
http://imgur.com/a/mQbYA
and after inverting the exposure and trying again:
http://imgur.com/aCaCTJJ

there is also chatter here once in a while:
https://groups.google.com/forum/#!forum/enzymaticsynthesis

> --
> -- You received this message because you are subscribed to the Google Groups
> DIYbio group. To post to this group, send email to diy...@googlegroups.com.
> To unsubscribe from this group, send email to
> diybio+un...@googlegroups.com. For more options, visit this group at
> https://groups.google.com/d/forum/diybio?hl=en
> Learn more at www.diybio.org
> ---
> You received this message because you are subscribed to the Google Groups
> "DIYbio" group.
> To unsubscribe from this group and stop receiving emails from it, send an
> email to diybio+un...@googlegroups.com.
> To post to this group, send email to diy...@googlegroups.com.
> Visit this group at https://groups.google.com/group/diybio.
> To view this discussion on the web visit
> https://groups.google.com/d/msgid/diybio/d79e9571-18c6-4b07-ac49-e8d2af93a0f0%40googlegroups.com.
> For more options, visit https://groups.google.com/d/optout.



--
-Nathan

[Shambo Hore]

  Thanks for your kind information