Illumina HiSeq 2000 obsolescence / salvage market : worth it?

[Ben Hunt]

I've been seeing Illumina HiSeq 2000s (their $10,000 genome machine from 2010) start flooding the salvage markets for pretty cheap ($hundreds on the local markets or $thousands on ebay and etc)

Turns out it is because Illumina stopped supporting them in February

Has anyone out there ever used them? Is there any reason to get them working v investing in minIONs and etc?

Thanks

[Brian Degger]

There is some nice hacking going on them by Gaudilabs/hackteria  ... Hackuarium also has had one. 

https://www.hackteria.org/wiki/HiSeq2000_-_Next_Level_Hacking  Lots of details on the components inside, and discussions of uses even if not as a sequencer. 

They seem like amazing pieces of kit 

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Brian Degger

twitter: @drbrian

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[Dakota Hamill]

Sequencers remind me of printers.  It's not the printer that gets you its the $1,000 a run cartridges and enzyme packs.  

But very cool if you can make sequencers DIY friendly! 

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[Brian Degger]

Apparently it also doubles as a cheap fluorescent microscope :) 

If you want optic systems, it contains a lot of high quality parts. 

On Mon, May 7, 2018 at 7:23 PM, Dakota Hamill <dko...@gmail.com> wrote:

Sequencers remind me of printers.  It's not the printer that gets you its the $1,000 a run cartridges and enzyme packs.  

But very cool if you can make sequencers DIY friendly! 

On Mon, May 7, 2018, 10:52 AM Ben Hunt <ben.g...@gmail.com> wrote:

I've been seeing Illumina HiSeq 2000s (their $10,000 genome machine from 2010) start flooding the salvage markets for pretty cheap ($hundreds on the local markets or $thousands on ebay and etc)

Turns out it is because Illumina stopped supporting them in February

Has anyone out there ever used them? Is there any reason to get them working v investing in minIONs and etc?

Thanks

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[John Griessen]

On 05/07/2018 01:27 PM, Brian Degger wrote:
> Apparently it also doubles as a cheap fluorescent microscope :)
> If you want optic systems, it contains a lot of high quality parts.

That "dissection" photo looks like a treasure trove of basic automation systems.
(Lausanne Bio-Hackerspace Hackuarium got a HiSeq2000 (SN# 700918) and Gustavo dissected it.)
https://www.hackteria.org/wiki/HiSeq2000_-_Next_Level_Hacking

[Skyler Gordon]

The optic systems are going to bounce lasers into your flowcell (glass slide) using a prism so even though you have a good detector, the light itself will be hindered unless you’re using Illumina fluorescent labels.

Dakota is right, it isn’t the sequencer it’s the reagents. Between that and the amount of clogged feed lines you would get on the HiSeq system, it most likely isn’t worth the money or the space it takes up.

Probably why people are getting rid of them.

-SG

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