Transfection in vivo - AAV vs PEI delivery efficiency

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Jan 4, 2021, 7:59:44 PM1/4/21

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Hi everyone!

Not something for DIYbio, but as there are a lot of smart people here, I was still wondering if anyone has done this.

We want to find a comparison of AAV to PEI for in vivo studies in mice.

~100 Million AAV particles with GFP are equivalent to XX micrograms of DNA packaged with PEI at the optimal ratio.

I guess it strongly depends on the PEI to DNA ratio; organism, injection site, ... etc. But maybe someone has seen numbers

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Jan 4, 2021, 8:02:33 PM1/4/21

to DIYbio

Maybe the easiest calculation would be like this:

They transfect x microgrms of DNA into cells and then it gets packged into 10^8 infectious replication-deficient AAV.