Autoclaving vinegar

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Andreas "Mega" Stuermer

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Mar 24, 2020, 11:53:18 AM3/24/20
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Hi everyone,

Growing some bioplastics right now.
Dissolving Pectin is really hard but autoclaving it is just magical.

Not, ideally I want it dissolved in 5% acetic acid ("table vinegar").

Flaming point is 38°C but ignition temperature seems above 400°C.

Is it smart to autoclave 100 or 500 mL of vinegar?

Just realizing how much better molecular biology is because there risk of anything exploding is practically zero

Andreas "Mega" Stuermer

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Mar 24, 2020, 11:56:17 AM3/24/20
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Where did the editing function go? 

"Not, ideally I want it dissolved in 5% acetic acid ("table vinegar")." = 
NOW, ideally I want it dissolved in 5% acetic acid ("table vinegar").

Cathal Garvey

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Mar 24, 2020, 12:46:37 PM3/24/20
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I suppose my worry would be whether the vinegar is better able to attack its container at 120°C, so maybe important to choose the right material..
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Rikke Rasmussen

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Mar 24, 2020, 3:16:38 PM3/24/20
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I think it might also be worth considering that vinegar is relative volatile, so your starting 5% might not be actual 5% afterwards. If you could get your hands on something more concentrated and filter sterilize that, then mix it with your pectin after you've dissolved it by autoclaving with just water, maybe that could work?

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Andreas "Mega" Stuermer

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Mar 25, 2020, 12:40:19 PM3/25/20
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Alright, thank you so much for your inputs!

Autoclaving it in water and then adding acid seems reasonable
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