Surface Plasmon Resonance

[Daniel Glenn]

I was wondering if their would be interest in an affordable surface plasmon resonance bio-assay sensor?

Surface plasmon resonance can be used as a method to detect antibody-antigen (and other) reactions directly with an optoelectronic sensor. Biacore and others make very expensive instruments that read 96-well plates, but I'm talking about something that reads samples one at a time and doesn't cost $33,000+.

It can be used to perform the same type of assays that occur in Western blot and ELISA without attaching tags, because the probe molecule is immobilized chemically on the surface of the optics with chemistry. Would those type of assays be useful to the general DIYbio community?

Do you think that there would be interest in such a device for the more general citizen-science community if test kits for things like Salmonella or Dengue that anyone with reasonable skills and training could use were developed?

[Dakota Hamill]

I have no idea what that machine does, but ask companies with millions of dollars if they'd buy it, not poor people like us.

As a story, someone I went to college with works at an antibodies company.  She and her boyfriend designed and welded a simple piece of metal that let's you stack western blot plates on top of one another on the orbital shaker.

She showed it to the higher ups and they wrote her a check for $10,000 and bought 100 of them..

Find a problem a company has, and fix it.  I personally don't know any DIYBio person who has a plasmond resonance issue, but there might be businesses out there that do.

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[Derek]

It's something I've thought about. I'd love to play more with it, but no longer have access to the chem lab with the appropriate hardware and don't really have the laser/optics expertise to do that in a cost efficient way. Could do some really neat assays with minimal ongoing costs if I had such a detector, though!

I do suspect Dakota is correct that you ought not base sales projections for a tool of that sophistication from this group. But I wouldn't have expected the raman spec to have caught on and had as much uptake as it has either, so if you have the capability to produce something that makes this technology affordable then it sounds like a great project!

[John Griessen]

On 01/25/2015 10:30 PM, Dakota Hamill wrote:
> Find a problem a company has, and fix it. I personally don't know any DIYBio person who has a plasmond resonance issue, but there
> might be businesses out there that do.

To use that with a detector that costs more than $1000 could get into a competition bind with this coming along for detecting
adsorbed molecules:

http://scitation.aip.org/content/aip/journal/apl/106/2/10.1063/1.4904481


On 01/23/2015 02:14 PM, Daniel Glenn wrote:> It can be used to perform the same type of assays that occur in Western blot and

ELISA without attaching tags, because the probe
> molecule is immobilized chemically on the surface of the optics with chemistry. Would those type of assays be useful to the
> general DIYbio community?

You didn't say at what price...

But, like Derek says, if you can get a spectrometer detector cost down under $200 it would go as a budget method.

The above linked new discovery detector, (surface -- but not plasmon resosnance), sounds like it
might sell for $1000 + $20 each supplies, or some kind of royalty
to make into lab-chips by 2017, so it could blow away your biz idea.

[Brian Degger]

there is a kickstarter for that (Surface plasmon resonance)

https://www.kickstarter.com/projects/1957476452/plasmonlab-surface-plasmon-resonance-biodetector

looks nice but needs a boost to get to the goal.

[On Kicktracker http://www.kicktraq.com/projects/1957476452/plasmonlab-surface-plasmon-resonance-biodetector/#chart-exp-trend]

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Brian Degger

twitter: @drbrian

http://makerspace.org.uk

http://transitlab.org

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[Maria Chavez]

We are working on an SPR community project looking into quantum biology at BioCurious in Sunnyvale.  We've had several meetings and are setting up a basic laser/optics setup this coming week I think.  What you are discussing is the sort of thing we want to investigate but not as a product more as learning how the technology works and building it ourselves in a DIY fasion.

-Maria

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[Derek]

Thanks, Brian, that is in fact the kickstarter of the person who started the thread. Cool! And backed. Whimper, my poor bank account. But I really want to see this go through.

(BTW. Daniel, good on you for not using the list for promotion of your campaign, but I sure am glad Brian found it and mentioned it. I hope you would have hinted that the campaign existed at some point, though!)

Derek

On Monday, 26 January 2015 10:57:41 UTC-8, DrBrian wrote:

there is a kickstarter for that (Surface plasmon resonance)

https://www.kickstarter.com/projects/1957476452/plasmonlab-surface-plasmon-resonance-biodetector

looks nice but needs a boost to get to the goal.

[On Kicktracker http://www.kicktraq.com/projects/1957476452/plasmonlab-surface-plasmon-resonance-biodetector/#chart-exp-trend]

On Mon, Jan 26, 2015 at 7:36 PM, John Griessen <jo...@industromatic.com> wrote:

On 01/25/2015 10:30 PM, Dakota Hamill wrote:

Find a problem a company has, and fix it.  I personally don't know any DIYBio person who has a plasmond resonance issue, but there
might be businesses out there that do.

To use that with a detector that costs more than $1000 could get into a competition bind with this coming along for detecting
adsorbed molecules:

http://scitation.aip.org/content/aip/journal/apl/106/2/10.1063/1.4904481


On 01/23/2015 02:14 PM, Daniel Glenn wrote:> It can be used to perform the same type of assays that occur in Western blot and ELISA without attaching tags, because the probe
> molecule is immobilized chemically on the surface of the optics with chemistry. Would those type of assays be useful to the
> general DIYbio community?

You didn't say at what price...

But, like Derek says, if you can get a spectrometer detector cost down under $200 it would go as a budget method.

The above linked new discovery detector, (surface -- but not plasmon resosnance), sounds like it
might sell for $1000 + $20 each supplies, or some kind of royalty
to make into lab-chips by 2017, so it could blow away your biz idea.

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[Nathan McCorkle]

Definitely interested. I'm wondering what sensor you were showing in
your images?

I've been studying low-noise design for high-noise environments such
as electron microscopes and quantitative analysis with various things
like spectrophotometry, potentiometry, voltammetry, electroporation,
x-ray spectroscopy and all the other noisy radio stuff in the
environment. It's always interesting to see new sensors!

If I can be of any help, let me know. I've got a few of these printed
circuit boards but haven't tried assembling them yet:
https://github.com/nmz787/open-spectrometer

On Fri, Jan 23, 2015 at 12:14 PM, Daniel Glenn <daniel....@gmail.com> wrote:

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[Josiah Zayner]

If Daniel responds to this thread I have a question about the Kickstarter.

Have you done any experiments to validate your instrument by showing it provides measurements similar to what is seen for commercial grade instruments in literature?

[Jacob Palumbo]

Sorry to necrobump a clearly dead thread, but I am actually specifically in need of this literally right now. In regards to Josiah's question, I'll run validation tests (can test lysozyme and various cancer biomarkers) in exchange for the spec and design files.

Let me know ASAP, thanks.

[Skyler Gordon]

I’ve never heard of SPR being used on liquids.

Usually SPR uses Infra-Red (IR-SPR) and anything that IR will bounce off of should give you a signal that represents that material. If your sample is negative without bonding and positive with, it sounds possible.

That being said, if you’re bonding something to a molecule that will result in IR-SPR signal, youre really just adding a probe anyways... I’d suggest just using some sort of probe.

-SG

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[Jacob Palumbo]

Here is a good paper on SPR as a biosensor: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4481982/

Contingent to my application with this device is the ability to test tens of things at once or more, meaning I can't use a single or even small set of probes. Plus as you may know, it is virtually impossible to attach probes to a specific protein in a raw lysate without also tagging a million other things. But SPR is a great way to find specific things in complex solutions label free using antibodies, etc.