Simple His-tag purification

[CoreyHowe]

A friend and I were talking about a simplified His-tag purification protocol for a peptide we'd like to produce and use, and I wanted to see what other people thought of it's feasibility. 

Instead of applying the cell lysate to a nickel column, first add nickel powder ($5 on ebay) to the lysate in a tube and mix, once properly mixed then apply a strong magnet to the outside of the tube to attract the nickel bound to the his-tag, pour off the excess lysate, lower the pH with an acid to elute the peptide from the nickel, and finally transfer to a new tube. 

Could this be feasible? 

[ukitel]

Simplified in which way?
You can do hig-tag purification without a column: with tubes and a centrifuge it works just fine

[CoreyHowe]

I guess I meant less expensive not more simple.

[Matt Champion]

This will not work. Ni metal (s) will not interact with high affinity on a His patch (his-tag). Nickel salts would in several cases (NiCl) but it is not magnetic

[Mega [Andreas Stuermer]]

What if you oxidize the surface of the particles with acid, while the core remains elementar Ni and magnetic?

[John Griessen]

On 02/16/2017 05:08 AM, Mega [Andreas Stuermer] wrote:
> What if you oxidize the surface of the particles with acid, while the core remains elementar Ni and magnetic?

sounds like a nanomaterial recipe idea:

1. Order nano nickel powder

2. Slosh with weak acid for ___ minutes

3. Rinse with DI water, dry.

4. Unclump it by some process like ball milling.

5. Package for sale in the mail.

6. Announce on diybio.

Anyone?
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Anyone?
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Joshua?