Kluyveromyces lactis - Anyone currently working with the yeast

[Andreas "Mega" Stuermer]

Hi, as the title says, wondering if someone had a sample to share

[Nathan McCorkle]

I know NEB sells it as part of their protein expression kit... as I got one once as a part of a bunch of free products I won from them, but never used it and it was left in a university freezer that I believe experienced a power loss later. Do we need to start a collection to get you started? I have some relatively easy projects (major assumption) that I was planning to use k.lactis as the main host, so I'm interested. I couldn't figure out a better way to keep the plasmid stable in subculture aside from using the acetamide selection NEB sells in the pKlac1 plasmid.

On Sat, May 26, 2018 at 2:34 PM, Andreas "Mega" Stuermer <andreas.t...@gmail.com> wrote:

Hi, as the title says, wondering if someone had a sample to share

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-Nathan

[Andreas "Mega" Stuermer]

I got two interested groups overseas, maybe 3. Overseas collaboration because GMO laws here don't allow indie research. Also have pKlac2 somewhere, we could have it amplified.

[Andreas "Mega" Stuermer]

Buying a commercial maxiprep is like 100$, split it into 100 tubes, and we can share with any collaborators. Will have to read the contract if this is allowed.

The yeast kit isn't cheap but it comes with 5 reactions. Have you used that system before?