I want to DIY a Stirred-Tank Bioreactor

[Priya Phalak]

hello group , I am new to DIYBIO .. I want to DIY a stirred-tank bio reactor,

tried going through https://github.com/Bioreactor/ but unable to catch a clue where to start it coz of geeked documentation

hence can some one demystify building bioreactor with simple step by step style document or help me find some other open source DIY Project

and guide me to build a bioreactor by providing every single details to DIY a bioreactor. 

kindly provide us the specifications of generic board numbers,specific board numbers  , sensors,  connectors , hardware etc.,

and also kindly provide us the materials like step by step documents and videos in this regard

with love

Priya Phalak

[leaking pen]

would you like us to hold your hand and wipe your butt for you too?

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[Lee Nelson]

I would start with a simple search.
https://www.google.com/search?client=ubuntu&channel=fs&q=stirred+tank+bioreactor&ie=utf-8&oe=utf-8

What are you tying to make, algae for fuel?

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[Maria Chavez]

I'd also check out this set of instructibles: http://www.instructables.com/id/Biomonstaaar/

Sent from my iPad

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[Raj Venkat]

Foolish irrational comment of self importance of mean racism.

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[Cathal (Phone)]

Having a bad day, are we?

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[leaking pen]

Sorry, geeked documentation?  The person basically said, i don't understand basic electronics, so i want someone to do this for me without myself doing the most basic research.  If they'd come in WITH some of that documentation, saying, I don't understand X Y and Z, i'd be HAPPY to help explain stuff.  But it seems we get these about once a week/month, requests to do the job FOR them, and I'm getting a bit sick of it. 

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[Cathal (Phone)]

And you don't imagine language and culture played a role in the formal and stultified email format?

If you don't like noobs, this isn't the right list for you. If you feel you're saying the same things frequently and plan to keep doing so, use a template instead of losing your cool.

[Mega [Andreas Stuermer]]

I think it's ok when fresh people want to learn science. Some of course will have no clue yet. But we shouldn't condemn them. Actually one causes intolerance against  diybio and science in general by repelling people. 

I also had no clue of practical biotech before getting the keywords here needed, and collecting the knowledge where to search for infos. Then I collected a decent amount of knowledge enabling me to design own experiments (with more or less success, strongly depending on my private budget situation) way before getting educated in University. And frankly, University didn't teah me much new biotech knowledge (just the enzymes in greater detail. And of course getting my hands dirty on wetware)

[Cathal (Phone)]

There are civil ways to decline an unreasonable request. Please just keep this list friendly and high quality.

On 21 January 2015 18:20:06 GMT+00:00, leaking pen <itsa...@gmail.com> wrote:

I didn;t say a thing about the stultified and formal email format, thats totally understandable as a language barrier. I complained about someone presenting a big job and asking for it to be done for them down to the smallest detail including sourcing of parts.  Sounds less like someone really trying to diy and more like someone getting other people to design a kit for them to makeand sell. 

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[Otto Heringer]

Assuming that is true she's being lazy or something, why botter!?

I just cannot understand someone activelly getting annoyed, spending all his ATPs to read and write something, just to express how annoyed he is.

How could you be so sure she is playing a intelectual dishonesty? Do you know her?

I hope to see the day when programming and electronics is a massively popular thing between life scientists - and when I say massively, I say in all the world, including countries in development. Im sorry Pen, but you just assumed this a commom thing, and is not.

Git hub is a messy repository for the one not used to it (there is even a nice initiative to teach how git works and how to use it - http://software-carpentry.org/).

A well documented source of information in a more friendly approach is the hackteria.org wiki. You shoul take a look. See, for instance, this page about a microalgae biorreactor: http://hackteria.org/wiki/Algae_Culture_at_Home

If you build this with your local materials and resources, please also share here the results <sarcasm> just to show how lazy you are </sarcasm>.

Free intolerance makes me mad.

Em quarta-feira, 21 de janeiro de 2015, Mega [Andreas Stuermer] <masters...@gmail.com> escreveu:

I think it's ok when fresh people want to learn science. Some of course will have no clue yet. But we shouldn't condemn them. Actually one causes intolerance against  diybio and science in general by repelling people. 

I also had no clue of practical biotech before getting the keywords here needed, and collecting the knowledge where to search for infos. Then I collected a decent amount of knowledge enabling me to design own experiments (with more or less success, strongly depending on my private budget situation) way before getting educated in University. And frankly, University didn't teah me much new biotech knowledge (just the enzymes in greater detail. And of course getting my hands dirty on wetware)

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[Erico Perrella]

Here (http://bit.ly/1xZuoaY) you can find a little bit of theory behind bioreactors.

Attached you can find some papers containing more detailed information about how to actually build them.

If you are interested in photobioreactors, then this website http://algaegeek.com/ have some nice projects you can take a look.

You can ask me directly in my email if you have any doubt, I am happy to help if I can.  

:)

On Wed, Jan 21, 2015 at 10:32 PM, Erico Perrella <erico.p...@gmail.com> wrote:

Here (http://bit.ly/1xZuoaY) you can find a little bit of theory behind bioreactors.

Attached you can find some papers containing more detailed information about how to actually build them.

If you are interested in photobioreactors, then this website http://algaegeek.com/ have some nice projects you can take a look.

You can ask me directly in my email if you have any doubt, I am happy to help if I can.  

:)

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[Dakota Hamill]

I have to drop out of the woodwork to agree with leaking pen.  I honestly thought the last 3 posts I saw to this group were trolls.  One wanted to do the Haber process in their kitchen, the second wanted a multi billion dollar biologic drug delivered in their garage, and the third asked for everyone on the list to do everything for them.  While I also can understand the after-posts, I can relate to the utter frustration of certain types of "gimmie gimmie gimmie" questions from people we don't know.  Some level of initiative should be shown, or people shouldn't be surprised by the type of responses they get.  Language plays a barrier, yes, but not in this particular instance I don't think.

I think now I understand why some of my professors just utterly snapped once in a while on the class as a whole.  I assume from their perspective their was this undeserved entitlement to laziness from some students, and if they all didn't get A's, it was the professors fault, when in actuality very few people overcame a challenge when met with one.

So, I'd say I completely relate to leaking pens frustration, and I'd also side with this being an "open and friendly list" but I don't see the need to call people out on it to seem morally superior in some way.   

If you ask a shitty question, expect a shitty response.

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[Raj Venkat]

The way Leakingpen puts it smacks of bounded irrationality rather than idiomatic usage of expression.Many support this idiotic usage ...talk of culture etc.Culture is self adaptive regulative mechanism of any individual or group. The moderator should warn such people.If a person asks a genuine Qs pl answer or remain shut up...using offensive language to lady cannot be tolerated by any group.I see many give wishy washy responses.This creates bad atmosphere universally.

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[John Griessen]

On 01/21/2015 08:36 PM, Raj Venkat wrote:
> The moderator should warn such people.If a person asks a genuine Qs pl answer or remain shut up...using offensive language to lady
> cannot be tolerated by any group.

I have to agree that no response is better than an unfriendly response.

[Lee Nelson]

https://www.google.com/search?client=ubuntu&channel=fs&q=stirred+biroreactor+kit&ie=utf-8&oe=utf-8

It seems that with all the brainpower here and the wikis that have been started we should have some great answers.
Especially for a project that is within DIY reach like a bioreactor.

Can we appoint a "hospitalitty coordinator" to help the people fresh to the list?

The tasks would include:

1: monitoring the list for new members or people coming out of lurking

2. helping them get the best information already on the list and an idea of where they can find more resources

 (Yes, that's right help the new people)

3. helping them organize their project like a mentor or project manager

     Timeline

     Bill of Materials

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[Cathal Garvey]

The first two are literally script-able: a bot that just observes for
heretofore-unseen "original posters" and replies them off-list with some
suggestions like "when asking questions, use a search engine first, then
check these community resources".

The same bot could then reply on-list with suggestions based on keywords
found in the OP's question.

The third is, I think, not our job. While a kind-hearted volunteer can
offer to help a noob organise a project, ultimately arranging stuff like
timelines, project management and BOMs is overkill for "being welcoming,
friendly and helpful".

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[Lee Nelson]

I agree about it being scriptable.

IRC chats have some nice bots that help the room with information like commands, pages and etiquette.

This list has been around for awhile.
I think we could be a bit more organized and cooperative and achieve more together.

Think of the list as a distributed hackerspace.

While we are on the topic of bioreactors, I have been thinking about waste management.
For tiny houses and boats.
https://www.google.com/search?q=tiny+house
https://www.google.com/search?q=tiny+house+bioreactor

http://tinyhouseblog.com/floating-homes/tiny-floating-homes-dos-libras/

Ideally this system could handle all of the collection, storage and heating of fresh water.
It would use use a cistern, solar heater and still.

Then the grey/black water is sent to the bioreactor.
The resulting biogas can be used for cooking and electricity generation.
https://www.google.com/search?q=biogas

What I need is the CAD file to make the house/boat 3D printable.
http://www.thingiverse.com/thing:519688
http://www.thingiverse.com/search?q=plumbing&sa=

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[Ravasz]

Trolling aside, there are a few people in this list, including me, who took part in building biomonstaaar, an open source bioreactor for algae. The instructable how to build it was posted earlier, you can find more info on it in the wiki:
https://sites.google.com/site/biomonstaaar/

If you have any questions, let us know.

[John Griessen]

On 01/22/2015 01:03 AM, Lee Nelson wrote:
> It seems that with all the brainpower here and the wikis that have been started we should have some great answers.
> Especially for a project that is within DIY reach like a bioreactor.

.
.
.

> Think of the list as a distributed hackerspace.

Sounds good. I'll be able to help with this some, but would rather not be point person for it.
I would have pointed to some write ups if I knew them myself, so helping people new to the list is already happening,
but there is little out there yet for bioreactors in instructables form except biomonstaaar.

Good idea to promote more of it though. It will become a side goal of mine to do write ups.
This reminds me of a man in Wales, (open mfrg list), that knows a lot
about practical bioreactors and conductivity sensors -- he hasn't got write-ups either.

I'm still working on web site infrastructure, much less adding daily to write-ups -- but I know writing will become essential:-)
I'm going to be asking Nathan M. some python django toolkit questions soon:-)
Maybe flask also...:-) A kickstarter campaign will be coming from me by May -- after much cheap talk about it for more than 2
years... That kind of thing needs focus since I don't have a budget otherwise. We know what happens when you lose focus
and take on too much -- nothing launched. So documentarian mode gets lower priority.

I agree with Cathal about the scope of "friendly diybio listmember" not including project management detail.

Technique on how to use FOSS bill of materials software, FOSS CAD, FOSS ECAD is in diybio's scope and on topic I think.

John Griessen

[Priya Phalak]

feeling like a freshman again after joining the group ... rather stiff welcome ... 

   lot of mixed feelings reading posts .... i neither want to debate racism .. elitist ... vandalism.. nor sympathy or support ....   

friends all i wanted is some support to decode that bioreactor  ... i thought this group is for diybio enthusiasts.. sorry if i misunderstood....                                                             i just wanna stick to my topic of discussion .. i am just in the pursuit of building a bioreactor  ...

with love

Priya Phalak

[Matt Lawes]

Generally folks are willing to help if you show some sign of sharing, giving back yourself. We all hate those who just take.

That plus this is do-it-YOURSELF biology. Asking for help and ideas, OK. Asking for everything, too much.

Best,

>matt

Sent from my Verizon Wireless 4G LTE DROID

Priya Phalak <dr.priy...@gmail.com> wrote:

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[John Griessen]

On 01/22/2015 11:21 AM, Priya Phalak wrote:
> friends all i wanted is some support to decode that bioreactor

OK. This is more specific maybe. Which bioreactor? biomonstaaar? --> https://sites.google.com/site/biomonstaaar/

[Nathan McCorkle]

On Thu, Jan 22, 2015 at 11:39 AM, John Griessen <jo...@industromatic.com> wrote:
> On 01/22/2015 11:21 AM, Priya Phalak wrote:
>>
>> friends all i wanted is some support to decode that bioreactor
>
>
> OK. This is more specific maybe. Which bioreactor? biomonstaaar? -->

Most likely the link to the one she posted in the first post.

[Nathan McCorkle]

On Wed, Jan 21, 2015 at 5:33 AM, Priya Phalak <dr.priy...@gmail.com> wrote:
> hello group , I am new to DIYBIO .. I want to DIY a stirred-tank bio
> reactor,
> tried going through https://github.com/Bioreactor/ but unable to catch a
> clue where to start it coz of geeked documentation

Yeah they didn't seem to do much documentation on github at least. I
believe these are the files for the electronics:
https://github.com/Bioreactor/Eagle/

but they used a non-open-source program (Eagle) and I can't easily
open them to take a look, and no PNG or JPG images it seems either,
for quick review. It would be nice if github would render the Gerber
files.

I recommend one of the other bioreactors that people here posted links
to... I am sure they should be better quality. Plus if you make some
progress, then get stuck, those people are active here to answer
questions.

Welcome to the mailing list, and please don't be offended by some of
the silly comments. We all need to start somewhere with learning this
stuff and getting comfortable, we all were beginners at one time, some
people just forget what it was like... and the frustration that goes
along with it!

:) happy bio-hacking!

[John Griessen]

On 01/22/2015 05:23 PM, Nathan McCorkle wrote:
> On Wed, Jan 21, 2015 at 5:33 AM, Priya Phalak<dr.priy...@gmail.com> wrote:
>> >hello group , I am new to DIYBIO .. I want to DIY a stirred-tank bio
>> >reactor,

>> >tried going throughhttps://github.com/Bioreactor/ but unable to catch a

>> >clue where to start it coz of geeked documentation
> Yeah they didn't seem to do much documentation on github at least.

No, I could not deduce any write up there after looking at headings and opening 15 files.
So it's undocumented code for an undocumented machine.

Hmm... the wiki on github has a gallery and some photos in it.

The first one is of a prototype made from acrylic with no pressure holding capability
and big enough to dunk a laptop in it.

The second photo is the same construction and shows a bench area of 1.4 sq meters
consumed by it all spread out as ethernet modules and power supplies and sensor
boards. A screen shot shows output code for holding constant temperature and varying volume.
No overall description of the code and multiple platforms and components anywhere.

Not much cost reduction engineering or miniaturization there yet.

To find out more would need asking the authors, who have not had much github activity since October.

[Will Canine]

Hi everyone! 

Just want to drop in my two cents about the broader discussion (sorry dont have something concrete to contribute re: bioreactors atm). Just a short story ;P

Two years ago, I was at a class in Genspace learning how to pipette and I was like "why hasnt someone made a DIY liquid handling robot yet? why cant I just download and run this protocol?" So I went to the DIY Bio listserv and looked around and, actually, someone HAD made one and even put it out on the listserv! So I reached out to them, and two years and a ton of work later, Chiu and Nick are my co-founders and OpenTrons is a reality. 

I think it is amazing that more and more people are looking to the DIY bio community for better, more accessible solutions to their research needs. Every "I wish I had a ______" email is an incredible opportunity to build a meaningful tool. And between the proliferation of accelerator programs / biohacker spaces and increased crowdfunding traction for DIY bio projects, developing and delivering these types of tools has never been easier. 

I hope that this community can rise to the opportunity and channel its talent, passion, and knowledge to build more amazing 21st Century biotech tools like Open qPCR (cant wait to get mine) and OpenTrons (cant wait to send ya'll yours ;). I think that many of us share the dream that DIY Bio's legacy will be a radically more horizontal and distributed bioeconomy, and I think building the tools that empower people to build better with biology is one of the best ways we can all make that a reality. 

</rant>

Also, thanks for all the great links on bioreactors! 

Thanks,

Will 

PS. If you are tryna bring your project to the next level, contemplating a Kickstarter, whatever, would love to share some hard-won lessons with you and chat. Just let me know! will at opentrons dot com. 

On Wednesday, January 21, 2015 at 8:33:36 AM UTC-5, Priya Phalak wrote:

hello group , I am new to DIYBIO .. I want to DIY a stirred-tank bio reactor,

[Priya Phalak]

yes i know its DO IT YOURSELF BIO and that's what my intent is also to DO IT Myself all i am asking is some instructables as i am a starter and as Nathan McCorkel rightly pointed out how could a starter have a licensed software like Cadsoft Eagle Professional.. well there are soo many challenges in it and i am willing to take them on...

 

my request to you all veterans is that if you could guide me guide me ... or else leave it but don't think i am not putting in any afford to DIY it.. and important thing is i am trying and not giving it up

with love

Priya Phalak

[Dan]

I have a Fisher glass bio reactor that is pretty simple. An aquarium type air bubbler plus heating element. We decided not to use it in an experiment  where we needed some hydrogen that was close to the LEL.  One of my buddies from LABiohackers made a bioreactor from a plastic cooler. Much  safer in case of explosion. I will try to track down the plans. I recall It did everything but wipe your arse. 

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[Cathal Garvey]

Now that it's public, I can chip in here that one of the indie.bio SF
teams is working on a cheap, open source bioreactor:
http://indie.bio/indiebio-sf-announces-first-class-11-biotech-startups/?utm_content=buffer6361d&utm_medium=social&utm_source=twitter.com&utm_campaign=buffer

(psst: Applications for Cork this Summer are open:
http://indieb.io/apply-to-ireland/ )

On 24/01/15 06:31, Dan wrote:
> I have a Fisher glass bio reactor that is pretty simple. An aquarium
> type air bubbler plus heating element. We decided not to use it in an
> experiment where we needed some hydrogen that was close to the LEL.
> One of my buddies from LABiohackers made a bioreactor from a plastic
> cooler. Much safer in case of explosion. I will try to track down the
> plans. I recall It did everything but wipe your arse.
>
> On Jan 23, 2015, at 7:46 AM, Will Canine <willc...@gmail.com

>> details to DIY a bioreactor*.***

>> kindly provide us the specifications of generic board
>> numbers,specific board numbers , sensors, connectors , hardware
>> etc.,
>> and also kindly provide us the materials like step by step
>> documents and videos in this regard
>>
>> with love
>> Priya Phalak
>>
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[Dakota Hamill]

Sensa is probably the company from the list I'm most interested in, because a bioreactor would be something I'd buy.  That's definitely a prototype I'd like to see!

How many of the companies have websites yet?  I've seen some random things from googling some of them but...

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[Priya Phalak]

these BIOSTAT® Qplus bioreactors I work in my university lab are a beauty ...

   wish DIYBIO comes up with such prisoned and  professional bioreactors

  which provide cutting edge support to researchers liked me who can

  hack hard the DIYBIO without bothering bout contamination or wastage

  

with love

Priya Phalak

[Lee Nelson]

Will Canine,

Thank you for your brilliant reply. That is exactly the kind of spirit I would like to see here. If we could get our act together, we could have something like YCombinator for bio.

As John Griessen mentioned, we could be using planning software. What if we could have smaller volunteer work-groups for different projects?

I would like to test this approach by nominating my 3D printed bioreactor restroom project for a workgroup. I have some knowledge of electronics, CAD and project management. The people who have already participated in the other bioreactor projects could be of great help.

The idea is simply for a low cost lavatory that would treat waste onsite and generate some energy in the process. There is a huge market for this product worldwide.

I have already started looking for references. I will set up some project infrastructure and post on my results for some high quality feedback.

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[John Griessen]

On 01/25/2015 12:49 AM, Lee Nelson wrote:
> I would like to test this approach by nominating my 3D printed bioreactor restroom project for a workgroup. I have some knowledge
> of electronics, CAD and project management. The people who have already participated in the other bioreactor projects could be of
> great help.
>

> The idea is simply for a low cost lavatory that would treat waste on site and generate some energy in the process. There is a huge

> market for this product worldwide.
>
> I have already started looking for references. I will set up some project infrastructure and post on my results for some high
> quality feedback.

Hmm.... the arid countries -- like west Texas -- can use such for clean environment and water reuse/conservation reasons.
What is the energy potential in grey water? I was thinking of a bioreactor as a net energy consumer usually.

It might have a good chance as a tiny low budget company product, since it lack's glamor so, those interested in IPO's
would not be much competition. With enough development time before big competition, a tiny company might
have a chance, even at a world market product.

[Lee Nelson]

It turns out there is a lot of money to be made in waste management.

Here is how the electricity can be generated.
http://scholar.google.com/scholar?q=electrode+electricity+bioreactor&hl=en&as_sdt=0&as_vis=1&oi=scholart&sa=X&ei=kgnFVIacBIrg8gXs6YDICw&ved=0CCsQgQMwAA

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[John Griessen]

On 01/25/2015 09:22 AM, Lee Nelson wrote:
> Here is how the electricity can be generated.

Hmm..the low power sensor net stuff I am developing aligns with those
microbial degradation electric systems...

"A new one-compartment fuel cell was composed of a rubber bunged bottle with a center-inserted anode and a window-mounted cathode
containing an internal, proton-permeable porcelain layer. This fuel cell design was less expensive and more practical than the
conventional two-compartment system, "

The anode sounds like the porous porcelain material used in hiking stream water purifiers...and you get to use cyclic
voltammetry to quantify your results -- fun!

[Lee Nelson]

I had thought that a battery and solar power should be part of the system.

This would drive LED lights and a fan.

I do not know if using a Joule Thief would be appropriate here.
https://en.wikipedia.org/wiki/Joule_thief

It could also have an Outernet receiver and phone charger.
https://www.outernet.is/en/

There is alot of good information from this search
https://www.google.com/search?q=toilet+bioreactor

I can see that all of the basic technology is in place it just needs to be combined and produced in a user friendly package.

It also occurred to me that most of this can be done with off the shelf parts and nod need 3D printing.

The real diy-bio part of this is the design of the tank or the protocol and control loop to keep this going.

I started a Gantt chart and sent an invite to the group.

Are there better ways to stay organized? Better online Gantt products?

I have seen many other, but do not know which is best yet.

Also I need open source CAD software that handles plumbing.

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[John Griessen]

On 01/25/2015 10:26 AM, Lee Nelson wrote:
> I started a Gantt chart and sent an invite to the group.
> Are there better ways to stay organized? Better online Gantt products?

Than Google?

> I have seen many other, but do not know which is best yet.

Me either. There was a sourceforge notice a while back
about a project that was active and popular...
"For our July Project of the Month, the community elected eXo, a highly customizable, social-collaboration enterprise platform. "
http://sourceforge.net/blog/july-2014-project-of-the-month-exo/

>
> Also I need open source CAD software that handles plumbing.

Handles as in piping diagrams? I doubt that exists yet, but
for an engineered, non-goofy-looking assembly, making models
of the PVC parts accurate enough they don't interfere
would be my idea, and that could take days, but then you
have those parts to locate in space, twirl assemblies
around on the screen, etc. I've used FreeCAD some,
and it's OK, but I'm studying BRL CAD again also now.
It's gotten some work in the last ten years -- Nathan said
it is usable, so I'm going to try too. It's one of my projects today even.

[Tom Hodder]

On Thursday, 22 January 2015 17:21:37 UTC, Priya Phalak wrote:

i am just in the pursuit of building a bioreactor  ...

You're not the only one!

openbioreactor.org is available lets do it!!! 

[John Griessen]

On 01/25/2015 11:00 AM, Tom Hodder wrote:
> i am just in the pursuit of building a bioreactor ...
>
>
> You're not the only one!
>
> openbioreactor.org is available lets do it!!!

I've had a list set up for a while...

_______________________________________________
Open_bioreactor mailing list
Open_bi...@lists.cibolo.us
http://lists.cibolo.us/mailman/listinfo/open_bioreactor

[Jeswin]

I think the solution is being over-engineered. Back in Uni., I came
across an old book called "The Toilet Papers: Recycling Waste and
Conserving Water" by Sim Van der Ryn in the back of the library. I
don't remember much, but the method he proposed was support of squat
toilets and use of sawdust rather than water. It made sense from an
environmental and health standpoint. I think it's a good read and it's
very short, especially in dealing with the area of waste disposal.
Sometimes solutions are made much more complex than they should.
Complexity of the method and expenses should be considered when
thinking of solutions.

Reading over the replies again, I think you guys have a different
endpoint in mind than than Van der Ryn. Well, all the best on your
project.

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[John Griessen]

On 01/25/2015 01:00 PM, Jeswin wrote:
> the method he proposed was support of squat
> toilets and use of sawdust rather than water.

Buckminster Fuller too. I thought we were talking about grey water, not black.
hand and body washing water. For desert dwellers. For near zero impact anyone.


As far as toilets, that's a big desert dweller deal also, and "sawdust-like"
compost filler material and exhaust fans and UV light and
heater elements and robotic dung beetles are what comes to mind.
Once that's perfected in the desert, maybe the rest will use it
to keep streams and ground water clean.

The black water is something
I put farther off and may never get to, but the grey water bioreactor seems
good for now...

[Priya Phalak]

wow amazing ... I wish I could catch hold of that "Open source bioreactors for every home" soon

with love

Priya Phalak

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[Priya Phalak]

this link is dead man 

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[Nathan McCorkle]

On Sun, Jan 25, 2015 at 9:02 AM, Priya Phalak <dr.priy...@gmail.com> wrote:
> this link is dead man
>
> On Sun, Jan 25, 2015 at 10:30 PM, Tom Hodder <t...@limepepper.co.uk> wrote:
>>
>>
>>
>> On Thursday, 22 January 2015 17:21:37 UTC, Priya Phalak wrote:
>>>
>>>
>>>> i am just in the pursuit of building a bioreactor ...
>>
>>
>> You're not the only one!
>>
>> openbioreactor.org is available lets do it!!!

I think they were saying it's available for a new website for a
project starting here/now :)

[Jonathan Cline]

On Wednesday, January 21, 2015 at 5:33:36 AM UTC-8, Priya Phalak wrote:

hello group , I am new to DIYBIO .. I want to DIY a stirred-tank bio reactor,

See the DIYbio FAQ where this topic is covered,  http://openwetware.org/wiki/DIYbio/FAQ


Everyone, the above sentence is the correct response to such questions.   RTFM yourselves at the link above.


## Jonathan Cline
## jcl...@ieee.org
## Mobile: +1-805-617-0223
########################

[Biotech Ryan]

Hi All,

In our first IndieBio SF class we'll have Sensa.io joining us, Greg (who worked out of Biocurious, Counter Culture Labs and most recently Genspace)  and the team are really passionate about building an open source bioreactor (they've build several bioreactors previously) and we're really pleased to be able to back him through IndieBio SF. I'm sure he'd welcome your thoughts and help with the open source bioreactor they'll be building to ensure it's as useful as possible to the community!

Feel free to reach out directly to him via Sensa.io!

All the best,

Ryan

On Saturday, January 31, 2015 at 9:28:36 PM UTC-8, Priya Phalak wrote:

If there is anything that depresses me more in this group is cynicism rather than debate, discussions, rebut or even arguments  over scientific topics..

people are inclining more towards plotting boundary , limitations of this group they are not doing any good to this group,

“Scratch any cynic and you will find a disappointed idealist... your cynicism wont stop my hunger for wisdom  ” 

 hmm no matter how much one may try to show his supremacy in literature, dialects or even as low as spelling  .. 

this is fkn bio group  discuss about bio only.. and your manuals wont stop somebody's passion..

On Wednesday, January 21, 2015 at 7:03:36 PM UTC+5:30, Priya Phalak wrote:

hello group , I am new to DIYBIO .. I want to DIY a stirred-tank bio reactor,

tried going through https://github.com/Bioreactor/ but unable to catch a clue where to start it coz of geeked documentation

hence can some one demystify building bioreactor with simple step by step style document or help me find some other open source DIY Project

and guide me to build a bioreactor by providing every single details to DIY a bioreactor. 

[Meredith L. Patterson]

On Thu, Jan 22, 2015 at 10:08 AM, Cathal Garvey <cathal...@cathalgarvey.me> wrote:

The third is, I think, not our job. While a kind-hearted volunteer can offer to help a noob organise a project, ultimately arranging stuff like timelines, project management and BOMs is overkill for "being welcoming, friendly and helpful".

Overkill for a human to walk newbies through every single time, to be sure ... but in keeping with your "the first two are literally scriptable," these aspects, too, have some automatable components.

The very first thing that comes to mind is MediaWiki templates. (Of which I wouldn't be surprised if there already are some for, say, BOMs, on OpenWetWare; Cedric and I did the inventory on the Biolab Brussels page directly into MW.) Ultimately it's too much work to handhold everyone through *populating* a project plan ... but, having tackled a large project in a new area to me (planning, scripting, and filming a video tutorial series for a software library I wrote) and gotten some help from a professional educational designer, I have to point out that forms help *a lot*, just in terms of keeping a lot of new ideas neatly organised.

The other thing this brings to mind is that if you are a parts vendor, building and hosting free design tools is the commoditise-your-complements pattern: your free thing brings customers for your actual business (parts) to your door. IDT customers might recognise this as the SciTools model.

Cheers,

--mlp

[Frantisek Algoldor Apfelbeck]

Hi to all,
I am happy to see some really nice input on the bioreactors/incubators in this tread.

With Food Hacking Base (fhb) group we are slowly progressing on our experimental incubator, nice PCB design and kit version will be ready for CCC camp in August (13-17th), it will be a third generation, second recorded respectively. So far we will measure and control temperature - heating and cooling, I hope also for humidity measurement but not control yet. We work with air with cultures in containers in the box simply put it, using peltier elements to heat and cool, but the later we may change because of variety of reasons. Info about the project is on our wiki, updates will be uploaded soon

https://foodhackingbase.org/wiki/Experimental_Incubator

Submersible or liquid based reactors/incubators are very interesting too especially with larger experiments and for people who are a lot into the brewing or liquid culture handling - warming air around the vessel when you can heat/cool it directly doesn't make too much sense.

If you are interested in updates or joining the effort please consider signing for you experimental incubator mailing list

===> send an empty email to subscribe to:  incubator-subscribe #you know the sign# lists.foodhackingbase.org

Sincerely,

Frantisek

[Priya Phalak]

hey Greg , keep  me posted with the progress in Indie Bio lab  about bioreactor i am looking forward to even actively participate and evaluate

your product even i can help you guys in functional testing as i have leverage of  experience particularly in suspension cell culture (using stir tank bioreactor )

and i have used several types of proprietary bioreactors in my view they all still relaying on obsolete  technology coz they are not designed in this generation i wish you

guys develop a  cross platform modular bioreactors which can be adopted for any purpose .. 

goo luck and keep me posted .. 

pls add me in your mailer list to receive updated or post updates in http://sensa.io

 

with lots of Love 

Priya Phalak

[Sunil Phani]

hey Priya , 

how far is your project moves ahead and how good is this opensource project  https://github.com/Bioreactor 

is that complected, or uncompleted or active and are there any active participants available to communicate  

and is there a strong  functional forum for this project.. you have to make sure about all these things before taking up 

an opensource ...

and add watch to this repository to get notification of critical updates 

any way best of luck for your project 

[leaking pen]

I didn;t say a thing about the stultified and formal email format, thats totally understandable as a language barrier. I complained about someone presenting a big job and asking for it to be done for them down to the smallest detail including sourcing of parts.  Sounds less like someone really trying to diy and more like someone getting other people to design a kit for them to makeand sell. 

On Wed, Jan 21, 2015 at 11:02 AM, Cathal (Phone) <cathal...@cathalgarvey.me> wrote:

And you don't imagine language and culture played a role in the formal and stultified email format?

If you don't like noobs, this isn't the right list for you. If you feel you're saying the same things frequently and plan to keep doing so, use a template instead of losing your cool.

On 21 January 2015 17:57:09 GMT+00:00, leaking pen <itsa...@gmail.com> wrote:

Sorry, geeked documentation?  The person basically said, i don't understand basic electronics, so i want someone to do this for me without myself doing the most basic research.  If they'd come in WITH some of that documentation, saying, I don't understand X Y and Z, i'd be HAPPY to help explain stuff.  But it seems we get these about once a week/month, requests to do the job FOR them, and I'm getting a bit sick of it. 

On Wed, Jan 21, 2015 at 10:50 AM, Cathal (Phone) <cathal...@cathalgarvey.me> wrote:

Having a bad day, are we?

On 21 January 2015 15:58:51 GMT+00:00, leaking pen <itsa...@gmail.com> wrote:

would you like us to hold your hand and wipe your butt for you too?

On Wed, Jan 21, 2015 at 6:33 AM, Priya Phalak <dr.priy...@gmail.com> wrote:

hello group , I am new to DIYBIO .. I want to DIY a stirred-tank bio reactor,

tried going through https://github.com/Bioreactor/ but unable to catch a clue where to start it coz of geeked documentation

hence can some one demystify building bioreactor with simple step by step style document or help me find some other open source DIY Project

and guide me to build a bioreactor by providing every single details to DIY a bioreactor. 

kindly provide us the specifications of generic board numbers,specific board numbers  , sensors,  connectors , hardware etc.,

and also kindly provide us the materials like step by step documents and videos in this regard

with love

Priya Phalak

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