Making anaerobic media

[ErazzyD]

Hello, I am trying to cultivate anaerobic bacteria in a DIY lab setting. Have any of you made anaerobic media? If so, what method of removing oxygen did you use?

[Skyler Gordon]

Are your bacteria strictly anaerobic?

-SG

On Tue, Nov 7, 2017 at 4:59 PM ErazzyD <jmoeller...@gmail.com> wrote:

Hello, I am trying to cultivate anaerobic bacteria in a DIY lab setting. Have any of you made anaerobic media? If so, what method of removing oxygen did you use?

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[wolass]

Use a Gaspack BD to get rid of air, and seal the bag in a thick foil that is not permeable for oxygen. You do not need a different medium just to get the air out of where the culture is. Alternatively you could use a glass bottle with a good seal and make a fluid culture, next remove the air inside the bottle by putting CO2 inside and quickly seal the bottle.

[ErazzyD]

They aren't strict anaerobes, no. Not like methanogens or anything. I think I can get away with a low oxygen, rather than fully anoxic, media. I am trying to batch culture it though, so I'm looking for something scalable. Plates and test tube liquid culture I have work arounds for, but I'm thinking of something that is feasible for 1L and up. There are lots of info online for this sort of thing presuming you have a full anoxic cabinet etc, but that's both out of reach in a DIY setting and overkill for what I'm working with.  

Thanks

[Skyler Gordon]

I’m not sure what your end goal is here, but what ever bacteria you’re using I would suggest thinking less about oxygen / non-oxygen environments and more about what electron acceptor you’re planning on using.

-SG

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[Abizar Lakdawalla]

In the "good" old days they used a burning candle inside a sealed container to lower O2 levels for culturing O2 sensitive bugs ... The candle would go off when O2 level dropped.

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