Why diy at 10cpb?cheap enough for 30c per aa. A 100aa peptide is $300 l (plus promoters etc)
--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diy...@googlegroups.com. To unsubscribe from this group, send email to diybio+un...@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+un...@googlegroups.com.
To post to this group, send email to diy...@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/bca73983-7b65-4787-a757-37688d4d561a%40googlegroups.com.
For more options, visit https://groups.google.com/d/optout.
What am I missing? I thought prices have been 10c a base for a while .....
Sent from my T-Mobile Android device
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/lqimg9hww9tmu00wt0qau4nt.1448156914306%40email.android.com.
Fair enough, but as a consumer I don't care how small or large their machine is. Quality and price are all I'm concerned about. And I recall 10c a base as being very standard pricing. Am I wrong?
Sent from my T-Mobile Android device
Anyone ever thought about what each bp costs?
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/lqimg9hww9tmu00wt0qau4nt.1448156914306%40email.android.com.
Ahh! That makes more sense. Where are companies like Gen9 coming in pricewise per bp?
>matt
Sent from my T-Mobile Android device
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/CAM%2BtiGJKRzJ%2B%2BBYj%2BpYrSOnOUPcCWSYtEBOtnohuCW5a3qd6ng%40mail.gmail.com.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/CAKw3Q72r2H%2BtzRjyvgRdJBGq5C7knpONPuXvFCsqmZqu0CYhcw%40mail.gmail.com.
gBlocks from IDT are ~$0.15 /bp. They go up to 2kb, and I've had good luck with them.
On Sun, Nov 22, 2015 at 1:31 PM Francis Lee <franci...@gmail.com> wrote:
maybe for oligos, but certainly not for gene synthesis.IDT ultramers which maxes out @ 200 bp go for ~ 0.16/bp if you order in bulk.Genscript is @ 0.35/bp for gene synthesisOn Sat, Nov 21, 2015 at 8:50 PM, Matt Lawes <ma...@insysx.com> wrote:
What am I missing? I thought prices have been 10c a base for a while .....
Sent from my T-Mobile Android device
On Nov 21, 2015 8:30 PM, Brian Degger <brian....@gmail.com> wrote:
Why diy at 10cpb?cheap enough for 30c per aa. A 100aa peptide is $300 l (plus promoters etc)
On 21 Nov 2015 23:27, "technologiclee" <technol...@gmail.com> wrote:
http://www.wired.com/2015/11/making-dna/--
"When Twist launches its beta program in 2016, it will offer gene synthesis at 10 cents per letter with a guaranteed turnaround time of 10 days."
"Moreover, the silicon wafer is cleverly optimized for the second step of gene synthesis—the stitching of oligos together—because Twist’s engineers figured out how to cut down on moving tiny volumes of liquid. Twist’s proprietary machine, a small-car sized system that WIRED wasn’t allowed to photograph, deposits one oligo into each of the 100 or so holes inside of a nanowell."
Is there any DIY system capable of this? What would it take to do that? What is the basic mechanism of moving the fluid in this system?
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diy...@googlegroups.com. To unsubscribe from this group, send email to diybio+un...@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+un...@googlegroups.com.
To post to this group, send email to di...@googlegroups.com.
Yeah. .. miscalculation on my part.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/a6c8fcc1-e612-435b-b6d0-fd9e9f5546b7%40googlegroups.com.
Way cheaper to just order them. You can get old oligo synthesizers for next to nothing if you look hard enough. But you can't buy the consumables for a reasonable price unless you want to start a company and do it full time. Even then you'd be using old tech and couldn't compete in the market.
--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diy...@googlegroups.com. To unsubscribe from this group, send email to diybio+un...@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+un...@googlegroups.com.
To post to this group, send email to diy...@googlegroups.com.
Visit this group at https://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/de7677c9-c87a-486b-acbd-3c720a16a916%40googlegroups.com.
On May 15, 2016 1:21 AM, "Jake" <jake...@mail.com> wrote:
>
> Way cheaper to just order them. You can get old oligo synthesizers for next to nothing if you look hard enough. But you can't buy the consumables for a reasonable price unless you want to start a company and do it full time. Even then you'd be using old tech and couldn't compete in the market.
Reagents and waste stream disposal costs are much too great to deal with unless you're going to utilize such a machine very often and have a steady stream of money coming in to pay for things. Acetonitrile, very-very-dry gas for flushing the reaction equipment and controlling valves (water looks like the hydroxyl on a nucleotide and terminates chain synthesis), and I haven't even got to the activation acid or a few other required items.
How I know? I have a synthesizer sitting unused for 2 years because I don't want to deal with all that annoyance (when all I want to do it is design and validation of genetic constructs).