Sterilizing plant hormones

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Andreas "Mega" Stuermer

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Nov 17, 2018, 4:49:24 AM11/17/18
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Hi guys, I need to make fresh stocks. The protocol calls for the hormone to be dissolved in 1M NaOH. And then filter sterilized.

My guess is that you can skip the filter sterilizing, because 1M NaOH will kill everything? Anyone with experience?

Google was remarkably unhelpful to give a yes/no answer

Rikke Rasmussen

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Nov 17, 2018, 1:55:51 PM11/17/18
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Some fungal and bacterial spores are remarkably hardy, and can conceivably survive 1M NaOH. I would go ahead and filter sterilize to be safe.

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Gerald Trost

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Nov 17, 2018, 1:58:39 PM11/17/18
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Hi Andreas!

you may ask the [PLANT-TC] group.

long time ago I made some PLANT-TC experiments in Klagenfurt
and I learned from a Netherland professional company that some
amoebe survived even hard autoclaving - they had to have it
tested by canadian specialists in order to find out.

In my naive view as a newby I think this is why many
microorganisms produce mucus - just to have some protected
encapsulation that cannot so easily be penetrated by acids and heat.

filter sterilization will not get rid of viruses but will hold back anything
larger than 15 micrometers - it will also hold back those cells
encapsulated in mucus - so my best guess is to obey the protocol.

yours, Gerald

 
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Sent: Saturday, November 17, 2018 at 10:49 AM
From: "Andreas \"Mega\" Stuermer" <andreas.t...@gmail.com>
To: DIYbio <diy...@googlegroups.com>
Subject: [DIYbio] Sterilizing plant hormones
Hi guys, I need to make fresh stocks. The protocol calls for the hormone to be dissolved in 1M NaOH. And then filter sterilized.

My guess is that you can skip the filter sterilizing, because 1M NaOH will kill everything? Anyone with experience?

Google was remarkably unhelpful to give a yes/no answer

Jeff Backstrom

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Nov 18, 2018, 1:08:58 AM11/18/18
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Prepare in 1M NaOH, then refrigerate. You can often co-autoclave with your media, depending upon the growth regulator. The other option is to prepare in ethanol, and add that.

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