I'm not sure what the clinical standard is for these tests, but I believe you could test for all of these with a PCR based assay. The basic equipment you'd need to get started are:
Pipettor(s)
Thermocycler (i.e. "PCR" machine)
Gel box and power supply
Gel light box
Look for this equipment on ebay or your local university's recycling store. You'll also need a bunch of consumable like tips, tubes, primers, polymerase, positive control DNA, agarose, DNA ladder, buffer salts, and dNTPs.
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Thanks Bryan! Sorry I'm such a newbie, but... Do I really need a lab space to do this? Meaning, a room in my house which is clean, sterile, temperature-stable? Or, if I'm careful (and maybe quick) can I get away with doing the PCR anywhere (let's say kitchen counter) as long as I clean the equipment and accessories thoroughly before and after use?
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As for the chlamydia I was treated for it recently and need to be retested. But being of a DIY mindset I thought, why not test myself? A PCR test costs $250 each time in the lab, and false negatives are a possibility. So I was thinking to keep retesting myself and my partner to see if it appears to be still there.
But it sounds like the primers are the biggest obstacle to this idea. Plus there are 20+ strains of chlamydia trachomatis, from what I've heard, so if the primer is too specific, it will generate a false negative I think?
Also I have zero lab experience/skills, and reading about the NAAT process it seems daunting. Of course the thermocycler does a lot of the work, but as a lab novice/newbie I'd be clueless on the most basic steps of the process, unless I had someone walking me through it in person. It's fun to dream and imagine the possibilities, though.
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That would be tricky. I'd be surprised if you could get the sensitivity & accuracy with a web cam and up light. But the bigger obstacle is that qPCR measures florescence between each of up to 40 cycles. So every couple minutes for a few hours you would have to pause your thermocycler, take out your samples, run to the dark room, take a picture, run back, put the samples back in the machine and unpause. Not impossible, but not exactly an efficient protocol.
Surely the only hard part of doing qPCR is getting the fluorescent agent? A standard webcam with a coloured filter over it and a uv light in a dark room should work no? Especially if you have a negative controle to measure background illumination against.
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The most common dye used is sybr green, which absorbs in the blue spectrum and, as the name implies, emits in the green spectrum.
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