Southwestern PA

Jo Donoghue

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Dec 8, 2016, 9:00:36 AM12/8/16

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Anyone around Pittsburgh or south of Pittsburgh want to start a group? I really enjoy bio but am looking for a group to assist me.

Josh Melnick

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Dec 13, 2016, 9:56:58 PM12/13/16

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Try to build one at Pittsburgh

Sean Donahue

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Dec 20, 2016, 3:26:41 PM12/20/16

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I might be interested. What specifically did you have in mind? I live a little south of Pittsburgh. I have some gear, and some friends who might be interested as well.

Jo Donoghue

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Jan 26, 2017, 10:11:03 PM1/26/17

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I am into genomics and want to dabble in plant genomics. I live near Uniontown, but I am willing to travel if you have a date and time in mind. Also do you have any thoughts on projects?

Sean Donahue

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Mar 7, 2017, 10:21:46 AM3/7/17

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Genomics is a little bit outside of my wheelhouse. I've worked mostly with molecular biology (PCR, electrophoresis, etc.) and microbiology. A couple months ago I tried the ODIN's CRISPR kit. My next intended project was Glowing Plant, engineering a bioluminescent houseplant with agrobacterium tumefacens. I ordered the supplies, but there was permits and paperwork involved, which isn't really my strong suit. I'm also working on building various equipment, a la Joshua Pearce's Open Source Lab. I'd be open to other ideas, though.

Jo Donoghue

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Mar 7, 2017, 7:16:59 PM3/7/17

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Cool sounds like fun how can I help you? Let's work with your ideas and go from there.

Mega [Andreas Stuermer]

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Mar 11, 2017, 11:38:41 AM3/11/17

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There are people who have successfully built gene guns for a few dozens of bucks. Avoids agrobacterium regulation

Sean Donahue

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Mar 23, 2017, 3:34:54 PM3/23/17

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My apologies for the lack of a timely response. I've had my hands full lately, but I should have more free time in the near future for side projects. If there's a way to avoid the agrobacterium related red tape, I'm open to it. I'd looked into a DIY gene gun several months ago, but hadn't found much. Perhaps I'll look again.