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How do the OpenPCR and the MJ Research machines compare?
The MJ machine cost us about 10 times more and has a bit more functionality. The advantages of the MJ machine has a higher capacity of 96 samples as opposed to 16 with the OpenPCR. The MJ can also do a temperature gradient so I can simultaneously test different annealing temperatures for different tubes. The MJ machine also did not require a computer. I think the MJ machine also has faster heat/cool speeds, so your run can be a little faster.
On the other hand, I like the OpenPCR interface a lot more. Since you use a PC, it's point and click, and type in the target temps and times. The stand-alone MJ machine uses an awkward interface with a hard to read display and unresponsive buttons.
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There's also NinjaPCR and GaudiLabs Wild OpenPCR, lightbulb pcr, and I believe the guys of syntechbio were also building their own design. So there is much to learn from and designs to try.
USB 3.1: Released in July 26, 2013, USB 3.1 doubles the speed of USB 3.0 to 10Gbps (now called SuperSpeed+ or SuperSpeed USB 10 Gbps), making it as fast as the original Thunderbolt standard. USB 3.1 is backward-compatible with USB 3.0 and USB 2.0. USB 3.1 has three power profiles (according to USB Power Delivery Specification), and allows larger devices to draw power from a host: up to 2A at 5V (for a power consumption of up to 10W), and optionally up to 5A at either 12V (60W) or 20V (100W). The first USB 3.1 products are expected to be available in 2016, and will mostly use USB Type-C design.
Forget this thread. I just received a message from Josh Preffeto that I am trying to cheat him by subverting Indian customs and making a personal profit. That just makes me sad.
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100 cycles | 12 °C | 60 s |
|
22 °C | 60 s |
|
12 °C | 60 s |
|
22 °C | 60 s |
|
12 °C | 60 s |
|
22 °C | 60 s |
|
12 °C | 60 s |
|
22 °C | 60 s |
Hold | 16 °C | infinite |
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My understanding is that PCR machines typically use solid metal blocks because they make it much easier to heat all the samples evenly and keep a steady temperature. The high heat capacity of the metal minimizes spacial and temporal fluctuations.
The cycler would just sit on the top of the washing machine when in use.If you need more than 50 degrees, you could turn up the house hot water temperature, or add a small inline water heater.
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## Jonathan Cline ## jcl...@ieee.org ## Mobile: +1-805-617-0223 ########################
I love the idea of a thin copper or aluminum plate stamped with tight-fitting dimples for the sample containers, and making a box with this as the lid, and running hot and cold water inside the box.
You get the temperature stability of a large water bath, and the rapid heat cycling without having to move the samples. Two large water baths kept at the two temperature extremes, and you just drain them through the box with the samples nestled in their dimples in the lid.
Spraying mineral oil or paraffin onto the sample containers before closing the lids also sounds like it would eliminate the need for heating the lids. So does the idea of simply filling the containers all the way to the top. No condensation if there is no vapor. The oil sounds a bit messy, though. At least paraffin is solid at room temperature. Why aren't the sample containers filled to the top? Even with distilled water?
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Voltage | Current |
6V | 160 mA |
7V | 190 mA |
8V | 220 mA |
9V | 240 mA |
10V | 270 mA |
11V | 300 mA |
12V | 320 mA |
a fast ramping air PCR thermocycler that needs no lid because it also
shakes the contents, (which also contributes to ramp rate performance)?
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Check it out. Uses what looks like squashed pipette tips as custom tubes. Peltier sand-which. Kinda like my wedge design. Apparently full 30 cycles in 11mins or so. Saw it in person. Crazy fast. My finger could barely keep up with the sensation of hot and cold when I kept it on the block!
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Hi,I recently decided to design a PCR machine that's affordable since I can't either buy one or afford an OpenPCR. I wanted your views on OpenPCR, MiniPCR and the likes of others. Are these runaway successes? Did everyone really buy one for themselves? What were the issues you had with them? What does diybio need today the most and at what price point.--ThanksUjjwal
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