Melting Peak

[Zirui Zhou]

I am trying to use immune-precipitation to detect histone modification (1 histone mark). But after I isolated CHIP-DNA and conducted real-time PCR, the melting peak of two positive luci showed a nearly straight line and the melting peak of the negative locus seemed normal. Could you give me some suggestions about how can this happen? I feel really confused because I cannot calculate relative fold enrichment under this circumstance. Thank you so much.

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Zirui Zhou

Ph.D. Student 

Department of Chemical Engineering 

392 Goodwin Hall, Virginia Tech