Should I plate them to plain media to let the shoot develop and grow regardless of the callus? Or if I plate it to a cytokinin, will the callus produce additional shoots despite already being on a plant?
I would grow the callus on plain media. MS basal salts, phytoagar, 3% sucrose, no hormones.
That *might* work but it will also mess with the seedling. So I would grow the seedling and callus separately if you want the seedling to continue growling normally.
I would grow the callus on plain media. MS basal salts, phytoagar, 3% sucrose, no hormones.So a callus will be able to self-differentiate into normal shoots without hormonal induction?
That *might* work but it will also mess with the seedling. So I would grow the seedling and callus separately if you want the seedling to continue growling normally.Out of curiosity, how will it mess with the seedling? I have some Drosera aliciae for example, seedlings that are much too small to separate from what appears to be developing callus cells.
That way you can split it up, expand it to cover more plates, etc.
One more practical question, must a callus be cut up? Or can the whole thing by dropped on induction media? It's one of those little things of basic technique I miss out on for not taking horticulture labs in school...
This is all great information, thanks for your help. I have a ton of the D. aliciae seedlings so I think I'm going to put them all in different media and see what happens. I have a kalanchoe as well, at first I thought it was cool, but now it's growing like weeds and I'm finding plantlets all over the patio!
I'm the one who brought the ants for K.G. at The Lab a few months ago if you remember. Hopefully I can become a member once I start getting into more advanced stuff!
If this isn't a callus, what could it be?