Re: [DIYbio] What is a efficient way to get lots of own my DNA?

[Jeswin]

I think the usual way for pain-free, hassle-free collection of DNA is by buccal swabs or saliva samples, which are then purified. Like you note, you will collect bacteria along with cheek cells. Maybe you should re-think the need for large quantity of DNA and find another way to show that C,H, N, O, and S are part of your DNA using less DNA? Sorry, I don't have a solution for you. Good luck.

On Sun, Oct 12, 2014 at 4:41 AM, Johann <joht...@gmail.com> wrote:

Dear DIY-biologists,

i need lots of my own DNA for experiments at school. The 'research' is about to find out using chemical detection reactions that carbon, hydrogen, oxygen, sulfur and nitrogen are the elements in my DNA . The method i used to get DNA does not produce enough. I used a method similar to this but with spiritus instead of pure isopropanol(worked better). The problems i have with this method are not only the small number of macro molecules i get, but also that i could have bacteria DNA in my sample. I am also lacking a method to dry the DNA.
So now my question:
Do you know a good way to get human DNA?
Good includes
 - nearly no bacteria DNA
 - Can do at home,( with chemicals from school lab)
 - Much DNA per Time
 - Can do in many iterations
 - No pain, for human subject :-)
 - My own DNA, no sample replication with nucleo acids, thermocycler or so
 - a procedure to dry DNA without destroying

Thank you for your inspirations and ideas

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[Dakota Hamill]

I don't really get what you're trying to do still.  Why do you want to reinvent the wheel?  

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[Patrik D'haeseleer]

Sounds like you might want to go back to the classics, and read up on...

Friedrich Miescher and the discovery of DNA

"On Hoppe-Seyler's suggestion, Miescher changed to examining leucocytes and obtained the cells for his experiments from the pus on fresh surgical bandages, which he collected from the nearby surgical clinic in Tübingen. In pus, he found the ideal base material for his analyses, and its “histological purity” allowed him to achieve the most complete purification of the chemical building blocks that constitute cells (Miescher, 1869a)."

Or, if you happen to lack a suitable purulent surgical wound, you could explore the second source of DNA that Miescher explored:

"Nonetheless, he worked on and discovered that sperm cells proved to be an ideal source material for the isolation of large quantities of very pure nuclein (Miescher, 1871c and Miescher, 1874b). Miescher chose these cells due to their simple composition with their heads comprised almost exclusively of a nucleus (Miescher, 1872a). Finally, he saw a possibility of obtaining sufficient amounts of nuclein to perform the exhaustive quantitative experiments he had already intended to do in Tübingen."

On Sun, Oct 12, 2014 at 4:41 AM, Johann <joht...@gmail.com> wrote:

Do you know a good way to get human DNA?
Good includes
 - nearly no bacteria DNA
 - Can do at home,( with chemicals from school lab)
 - Much DNA per Time
 - Can do in many iterations
 - No pain, for human subject :-)
 - My own DNA, no sample replication with nucleo acids, thermocycler or so

Yup, I think we've got all those criteria covered. Good luck with your school project!  ;-)

Patrik

PS: the use of salmon sperm DNA in molecular biology (e.g. as a carrier DNA when doing transformations in yeast) dates all the way back to 1871 with Miescher! The things you learn when researching vegan alternatives to standard lab protocols...