Using bubblewrap to create chemical and bi assays...

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André Esteves

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Jul 18, 2014, 9:08:19 AM7/18/14
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Make a frame to hold the bubble wrap and put it in a 3d printer with the printing head replaced by a programmable syringe and you can do all of combinatorial interesting things...

Apply nail polish with a brush over the holes and you are done...

www.newscientist.com/article/dn25916-bubble-wrap-used-for-cheap-blood-and-bacteria-tests.html?utm_source=NSNS&utm_medium=SOC&utm_campaign=facebookgoogletwitter&cmpid=SOC%7CNSNS%7C2012-GLOBAL-facebookgoogletwitter#.U8ka-h_HnFw

Cheers,

André Esteves

Nathan McCorkle

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Jul 18, 2014, 12:31:28 PM7/18/14
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It would suck if you got some bubble wrap contaminated with spores.

I use a heat sealer to package stuff in a clean room, and the plastic roll we use was certified manufactured in a cleanroom too. I wonder if that grade of bubble wrap is produced, and what it costs.

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Sebastian Cocioba

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Jul 18, 2014, 1:35:34 PM7/18/14
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Can someone try it with some SOC media and inccushake it at 37c? Im guessing the air or plastic is hot when made so whatever is trapped had a good chance of dying. Spores may resist. Clean room bubble wrap sounds freaky-expensive.

Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D
From: Nathan McCorkle
Sent: ‎7/‎18/‎2014 12:31 PM
To: diybio
Subject: Re: [DIYbio] Using bubblewrap to create chemical and bi assays...

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SC

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Jul 18, 2014, 2:24:16 PM7/18/14
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Just curious, why would this be better than a regular culture/assay plate?  Or was the original intent to save money?

André Esteves

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Jul 18, 2014, 2:27:39 PM7/18/14
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i think the intent of the repurposing,  was price for 3rd world countries. It's a fraction of the $3 dollars price for a plastic assay. Read the news in new scientist and the original article.

Cheers,
André Esteves


2014-07-18 19:24 GMT+01:00 'SC' via DIYbio <diy...@googlegroups.com>:
Just curious, why would this be better than a regular culture/assay plate?  Or was the original intent to save money?

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Jonathan Cline

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Jul 18, 2014, 2:34:59 PM7/18/14
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If a 3d printer is already available, why not just print 384-well microtiter plates..

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Nathan McCorkle

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Jul 18, 2014, 3:15:53 PM7/18/14
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Most of the multi-well plates are sterilized with Gamma radiation... I
guess maybe HEPA filtered air made into plasma could help, but
diffusion into tiny wells seems like it would take a while.

I'd be concerned with FDM printer stock being unclean too. And it
could vary batch to batch, trace contaminants would be a PITA to track
down.

I'm just making the worst of this though, sterility could be fine and
dandy, and maybe the plastics/contaminants benign enough. Not too
interesting to me though, I wouldn't want a mis-diagnoses because I
couldn't get the more pure plastic or more clean plastic.
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Simon Quellen Field

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Jul 18, 2014, 3:17:39 PM7/18/14
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UV or gamma sterilization may also work.
Or you could fill the syringe with bleach, fill the bubble, suck it back out, rinse with sterile medium, and inoculate. It adds time to the procedure, but its automated.

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André Esteves

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Jul 18, 2014, 3:18:19 PM7/18/14
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Have you ever printed something? It's not fast and using supplies for throw away stuff it's not rational.
Imagine that you recycle your plastic... Are recycling contaminated stuff?

Bubble wrap can just burn and with it take away the baddies...

Cheers,

André Esteves


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Simon Quellen Field

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Jul 18, 2014, 3:19:51 PM7/18/14
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The New Scientist article says the inside of the bubbles is sterile.
Sounds like an easy test to run.


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Jonathan Cline

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Jul 18, 2014, 3:35:14 PM7/18/14
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Lasers.  Clearly someone needs to try lasers.  Nanotechnology lasers.

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On 7/18/14 12:16 PM, Simon Quellen Field wrote:

Jonathan Cline

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Jul 18, 2014, 5:10:51 PM7/18/14
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Three Dee Print a reusable well frame (mold) and vacuum/heat form 0.2 mm LDPE film or saran wrap into the wells.   3d printing of plates directly has previously been discussed here https://groups.google.com/forum/#!topic/diybio/4gzh_PP_hd8    Yes, microtiter plates are reusable, depending..


You burn your discarded bubble wrap?


The upside of using bubble wrap, obviously, is that the bored biologists can pop the extras.


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On 7/18/14 12:18 PM, André Esteves wrote:

Nathan McCorkle

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Jul 18, 2014, 5:28:02 PM7/18/14
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/me envisions some over-excited lab tech jumping on and popping bubble
wrap, until another tech walks in 'my experiments!'
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Sebastian Cocioba

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Jul 18, 2014, 5:50:16 PM7/18/14
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Next shipment that comes with decently sized bubble wrap I get ill
squirt some luke warm MS-Agar, let it solidify, and dispense a few
Arabidopsis seeds atop. Wonder what smallest needle gauge to still pass
seeds?

Nail polish should work to seal it, right? Would be interesting to try.
I use eppi tubes to start small seeds for quick DNA processing etc and
reuse the tubes by autoclave. Quick disposable titer plates for testing
mannose isomerase activity would be nice. Just an overnight
colorimeteric assay of small bit of callus tissue.

As for saran vacuum forming, how many sheets do you guys think would be
enough for a decent replica of a standard titer plate?

Ill post pictures if I happen to stumble upon some fun...err...bubble
wrap :P

Very fun ideas, everyone!

Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D From: Nathan McCorkle
Sent: ‎7/‎18/‎2014 5:27 PM
To: diybio
Subject: Re: [DIYbio] Re: Using bubblewrap to create chemical and bi
assays...
https://groups.google.com/d/msgid/diybio/CA%2B82U9KKy1kOydYsfe%2BA4by6PK1z1mJrc6ej10YOavCoJfKY9g%40mail.gmail.com.

Jonathan Cline

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Jul 18, 2014, 6:10:35 PM7/18/14
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Nail polish is not bio-friendly stuff.  In fact, quite toxic.  (I question why anyone would use it on themselves..)  Try a small bit of scotch tape, maybe use a hole punch to easily cut & size the tape.

It is a little funny that the original article presumes bubble wrap is a good solution because it's freely available " for labs around the world with limited resources" then suggests nail polish. Which, clearly...



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On 7/18/14 2:50 PM, Sebastian Cocioba wrote:
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