Re: [DIYbio] Where to get a micropipette for nuclear transfer/injection ?

[Nathan McCorkle]

Probably just look for a syringe pump

On Jun 3, 2013 10:17 AM, "Le Minh Duc" <ducfa...@gmail.com> wrote:

Hello,

I searched in Ebay with the keyword micropipette but didn't found any pipette for nuclear transfer / ICSC injection pipette.

Any one know where to buy it ?

Thanks

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[Josiah Zayner]

Are you talking about holding the cell in place? or the the needle for the injection? The pipetting mechanism?

Try microinjection pipette.

People tend to pull their own pipettes or use machines to pull them.
http://www.ncbi.nlm.nih.gov/pubmed/22484674

[SC]

They're easy to make.  Get a package of glass capillary tubes, hold each end with your fingers, move the middle over a gas flame, and pull slowly.  Each end becomes a micropipette.   You may need to practice a bit to produce ends that are an appropriate diameter for your intended use.

 

 

[Koeng]

I found this

http://www.ebay.com/itm/Eppendorf-Transjector-5246-Microinjection-System-/170858429811?pt=LH_DefaultDomain_0&hash=item27c7f4bd73