Cultivation of bacteria

[Michael Jancen-Widmer]

Hi everybody!
I wanted to learn the basics of DIY-Bio and so I started to cultivate bacteria that grow on my hand. After my 4th attempt there is still no result. I used gelatine and distilled water.

[Dakota Hamill]

put in a bullion cube, or a little bit of sugar.  you need some salts and nutrients, not just water and a gelling agent

On Sat, May 30, 2015 at 1:18 PM, Michael Jancen-Widmer <jancen...@gmail.com> wrote:

Hi everybody!
I wanted to learn the basics of DIY-Bio and so I started to cultivate bacteria that grow on my hand. After my 4th attempt there is still no result. I used gelatine and distilled water.

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[Mega [Andreas Stuermer]]

You should check if it is legal in your country. There are a lot of biosaftey-2 organisms growing on your skin. Welcome to biolaw...

Maybe you want to buy LB agar and sterilize it in marmelade jars? Would cost more but less messing around finding the right ingredients

[Michael Jancen-Widmer]

Thank you for your feedback. I'm from Germany and I think it's legal but illegal to modify them.

On 4 Jun 2015 19:03, "Mega [Andreas Stuermer]" <masters...@gmail.com> wrote:

You should check if it is legal in your country. There are a lot of biosaftey-2 organisms growing on your skin. Welcome to biolaw...

Maybe you want to buy LB agar and sterilize it in marmelade jars? Would cost more but less messing around finding the right ingredients

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[Nathan McCorkle]

On Thu, Jun 4, 2015 at 11:08 AM, Michael Jancen-Widmer
<jancen...@gmail.com> wrote:
> Thank you for your feedback. I'm from Germany and I think it's legal but
> illegal to modify them.

It is also recommended to sterilize the media /after/ growth but
/before/ throwing it away. This is in case any of the pathogens that
may have (likely) grown, you don't get an infection when you are
scooping the gelatin/agar into the trash can.

> https://groups.google.com/d/msgid/diybio/CAO%3D5TEhTLamzpTjmccJ2h%2Ba9xh2Pqv-3UF5Ds_GNCOi7BGgsyA%40mail.gmail.com.

>
> For more options, visit https://groups.google.com/d/optout.

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-Nathan

[Michael Tellier]

I would also recommend LB-agar medium poured into a disposable recipient (Petri dish) which should remove the risk of infection since you throw away everything and not just the media. However, the disposable recipient might still contain pathogens. 

I did some trials for skin bacteria and I got a few different species/strains on LB-agar. I still need to sequence their 16S to determine their species/strains. None of them grew in liquid culture though. 

The major problem with working on bacteria found on humans is the possibility of airbone infections since it is quite difficult to know with what you are working with.