used cell incubator

[Frank]

Hi everyone

A while back I requested help defining a protocol for a therapeutic adipose derived stem cell procedure that I would like to perform. Unfortunately I didn't get as much feedback as I had hoped so I had to go ahead and choose an approach myself. I am now working on the protocol . I think I know all the materials I need except of course a cell culture hood or a proper laboratory to work in given that,amazingly, in New York City there isn't a biospace available . So I'm going ahead with it anyway and I think the only significant expense will be one piece of equipment. I'm not using Calf serum, substituting autologous platelet lysate. The missing piece I think is a cell incubator . I know there are many for sale used. But I have no idea what type, size, etc.  

I would love it if someone can tell me the minimum features I need for that incubator . All the information about what I'm doing and the materials list alright here on this working document that I posted in Google Docs. 

https://docs.google.com/document/d/1GYuNYQ4EdmUAI5Ljq6TTxKzE2J5ea6kR07Bo7ul08kY/

[Nathan McCorkle]

I thought there were two community biolabs in Brooklyn (genspace, biotech without borders), and also a decent DIY BIO community.

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[Frank]

BWB closed up and is currently formulating a plan to reopen in the future (not immediate). Genspace told me they are not equipped to do my experiment. 

To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/CA%2B82U9JxoUX%3D2o8Lqs-iWZ_7JBU%2BY8mDeATFZnVQAv8q8P0tzg%40mail.gmail.com.