Efficient homologous recombination design

[Mega [Andreas Stuermer]]

Hi everyone... 

Could such a homologous design as in the picture possibly work? 

The difference: a loop would have to be formed. 

Thanks a lot! 

[Nathan McCorkle]

My guess is it'd be a transient species that wouldn't last very long,
unless the loop was designed to fold onto itself really well, or
somehow wrap around the flanking regions.

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--
-Nathan

[Mega [Andreas Stuermer]]

I thought as soon as it forms crazy holiday junctions it could be stably integrated ito the bacterium's genome at the chosen site?