Safely disposing of old cultures?

[MC]

For those of us without access to biohazard waste receptacles, how do you safely dispose of old cultures? For example, non-pathogenic E. coli (BL21, DH10b). Is it safe enough to just toss in the trash? Or what is an effective way to ensure sterilization (in non-autoclaveable plates) before heading to the trash?

[Rikke Rasmussen]

Make a 10% solution of household bleach in a bucket, toss them in there overnight.

On Mar 4, 2017 9:49 AM, "MC" <marky...@gmail.com> wrote:

For those of us without access to biohazard waste receptacles, how do you safely dispose of old cultures? For example, non-pathogenic E. coli (BL21, DH10b). Is it safe enough to just toss in the trash? Or what is an effective way to ensure sterilization (in non-autoclaveable plates) before heading to the trash?

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[Mega [Andreas Stuermer]]

AFAIK all trash (Restmüll - residual trash) in my country is brunt to get energetic value. 

We have 4 containers - paper trash, plastic trash and bio trash and residaul trash (everything else). I would assume non-modified non-pathogenous coli should be fine to go into trash. People trow molding bread in there and molds may actually poison you with mycotoxins and allergens etc (http://news.nationalgeographic.com/news/2005/05/0506_050506_mummycurse.html). 

If you heat kill it in a pressure cooker at 121°C; 20 mins that would be good practice though

[Nathan McCorkle]

On Tue, Mar 21, 2017 at 1:54 PM, Mega [Andreas Stuermer]
<masters...@gmail.com> wrote:
> If you heat kill it in a pressure cooker at 121°C; 20 mins that would be
> good practice though

That's what we did at school with an autoclave.

I won't cook such things in my main for-food kitchen though. Just my
paranoia about volatilizing weird stuff that might have grown.
Also, around here I feel like pressure-cooking outdoors on the porch
might disturb the neighbors. So I will need to do it during the
daytime, at least, if I don't think of a better solution soon.

I just bought a CO2 laser cutter though, which also needs exhaust, and
my spin-coater could use exhaust too. So I might just be on the
lookout for sheet metal and try building something of my own, for the
garage or something.