Spin column contamination article

[Avery louie]

http://www.virology.ws/2013/09/18/de-discovering-pathogens-viral-contamination-strikes-again/

Tl,dr; silica columns contaminated with virus, causes false positive.  Pretty interesting, thought you all would enjoy it if you have not seen it.

[Jeswin]

Here's 2 articles that I looked into a few years back when my lab was dealing with contamination. As I recall, we had qPCR experiments with unexpected positives. I believe I also got positives in purification column controls. I forget how it got resolved but I'm not surprised you get contamination during the manufacturing process.

Evans, Gloria E., et al. "Contamination of Qiagen DNA extraction kits with Legionella DNA." Journal of clinical microbiology 41.7 (2003): 3452-3453.

Tilburg, Jeroen JHC, et al. "Contamination of commercial PCR master mix with DNA from Coxiella burnetii." Journal of clinical microbiology 48.12 (2010): 4634-4635.

On Mon, Mar 23, 2015 at 10:26 AM, Avery louie <inact...@gmail.com> wrote:

http://www.virology.ws/2013/09/18/de-discovering-pathogens-viral-contamination-strikes-again/

Tl,dr; silica columns contaminated with virus, causes false positive.  Pretty interesting, thought you all would enjoy it if you have not seen it.

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[Nathan McCorkle]

neat.

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