Z-competent cells

[Joseph Chemler]

Does anyone have a guess at the technology behind Zymo Research's Z-competent cells? (It's proprietary)

They do not require heat shock and are super easy to make. Probably the easiest plasmid transformation procedure I have ever used.

[Nathan McCorkle]

My bet is on PEG and MgSO4, as laid out here:
'Easiest transformation method?':
https://groups.google.com/forum/#!topic/diybio/bXiyu_ZW9H4

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[Joseph Chemler]

That looks to be it. http://www.pnas.org/content/86/7/2172.full.pdf
Nice. Thanks!

[Mega [Andreas Stuermer]]

Proprietary? 

Cathal's been using that for years :D 

[Dakota Hamill]

So have many people

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[Cathal Garvey]

I'm told it's Tom Knight's "Second Favourite" method. Nobody told me
what his favourite method is. I assume it's just glaring at recalcitrant
cells until they transform themselves.

>> https://groups.google.com/d/msgid/diybio/cbc41dc2-8081-4c7c-9aec-aa1b0a7f3ca0%40googlegroups.com<https://groups.google.com/d/msgid/diybio/cbc41dc2-8081-4c7c-9aec-aa1b0a7f3ca0%40googlegroups.com?utm_medium=email&utm_source=footer>
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[Tom Knight]

This is my favorite technique:
http://openwetware.org/wiki/TOP10_chemically_competent_cells
I didn’t document the part about glaring at them.

> <0x988B9099.asc>

[Cathal Garvey]

Ah, I remember when I worked somewhere with enough money to burn on
Top10s! :)
Good times, and also where I swiped my first culture of plasmid-free DH10B.

Thanks for the answer!