HPLC Designs Utilizing Microbore Tubing

[Cory Geesaman]

Anyone know offhand of some HPLC designs that utilize microbore tubing?  I've been looking into the concept lately and it seems like using some of the larger (~1mm ID or larger) sizes could be feasible with capillary tubing for the detector and a peristaltic pump to keep it all contained (maybe just using multiple peristaltic pumps to eliminate the need for valves which seem to be very expensive even on places like eBay).  Considering PTFE microbore tubing but might need to go with something else for pump tubing, any suggestions on this topic welcome.

[Josiah Zayner]

All you really need for an HPLC is a column, a pump/valve assembly and tubing.
All of this can be purchased on eBay for < $100. I purchased multiple working HPLC pump/valve assemblies on eBay for < $50 including shipping.
Just troll eBay for a bit you will find something.

[Nathan McCorkle]

You'd wouldn't be able to pack as much media (solid phase) in the smaller column, so you couldn't use as high concentration samples (at a certain concentration the column will saturate and cause wide bands and possibly overlapping peaks) or you'd need to inject less volume. You might have lower detection sensitivity too, if you're looking for an absorbance reading.

Google has lots of results for microfluidic or microbore hplc

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[Nathan McCorkle]

Also the pressure might go up if you want the same separation time as with a big hplc.

On Aug 18, 2013 10:48 PM, "Cory Geesaman" <co...@geesaman.com> wrote:

Anyone know offhand of some HPLC designs that utilize microbore tubing?  I've been looking into the concept lately and it seems like using some of the larger (~1mm ID or larger) sizes could be feasible with capillary tubing for the detector and a peristaltic pump to keep it all contained (maybe just using multiple peristaltic pumps to eliminate the need for valves which seem to be very expensive even on places like eBay).  Considering PTFE microbore tubing but might need to go with something else for pump tubing, any suggestions on this topic welcome.

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