Deinococcus

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Linden

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Aug 30, 2015, 8:52:45 PM8/30/15

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Hey guys!

Has anyone ever worked with Deinococcus, and know where I could find DNA manipulation protocols for this organism?

Thanks!

Mega [Andreas Stuermer]

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Aug 31, 2015, 8:26:07 AM8/31/15

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http://www.researchgate.net/publication/224812866_Development_of_versatile_shuttle_vectors_for_Deinococcus_grandis

I googled for "D. Plasmid", often they characzterize minimal plasmids by cutting them and seeing what replicates in the species.
Worst case you can always try carbon whisker or electroporation

Linden

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Aug 31, 2015, 7:45:28 PM8/31/15

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Thanks, I will try that ;)

Linden

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Aug 31, 2015, 7:46:32 PM8/31/15

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