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On Oct 5, 2013 2:45 AM, "Mega [Andreas Sturm]" <masters...@gmail.com> wrote:
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> There is a protein called monellin. It is said to be much, much sweeter than sugar.
I heard a talk by Cynthia kenyon on c elegans longevity research and it showed that even olfactory stimulation by sweet stuff caused an expression response that could eventually lead to similar insulin resistance scenarios (type 2 diabetes).
So it seems moderation is the key to anything sweet regardless of caloric density. That said, seems sugar is a simpler solution if non-calorie sweeteners don't actually taste more /interesting/ than common sugars. Things like miraculin are cool, sugar substitutes aren't nearly as exciting.
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I believe your idea could make GMOs more acceptable.People see your yoghurt and it says "makes you live longer and healthier" and "contains GMO derived products". Then they have to choose. And even the most (pseudo-)environmentalist wants to live a longer healthier life...
So, many people will choose GMO if it brings health benefits (instead of making it insecticide producing)
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Carolina.com sells e.coli ampicillin and a plasmid for $24, presumably plus shipping.
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Very cool project! I'd love it if you guys could come give a talk about your work at Counter Culture Labs - your friendly neighborhood DIYbio group. You're right in our backyard after all! This is just the kind of projects we'd love to get into once we get our lab up and running.
Do you foresee any trouble synthesizing the p-coumaric acid in lacto? The high-throughput E. coli resveratrol biosynthesis papers I've seen (e.g.
High-Yield Resveratrol Production in Engineered Escherichia coli - 2.3 g/liter resveratrol!) all seem to feed in p-coumaric acid, because getting the C4H enzyme to work in bacteria seems problematic:One of the barriers to the production of flavonoids and their related compounds in microorganisms by means of assembling biosynthetic genes to form an artificial pathway is the difficulty in expression of active, membrane-bound C4H. This enzyme is not expressed efficiently in bacteria due to its instability and the lack of its cognate cytochrome P450 reductase in the host.
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I may have been a bit misleading in the last email, as I was ranting more about mutagenesis as it refers to 'shotgun genetic engineering' aka using chemo and radio mutagenesis. With Synthetic Biology *wink wink*, one could simply use a computer program to mutate nucleotide by nucleotide the protein's gene. After a while it might just happen to look like a heterologous gene, but assuredly it wouldn't be cross-species, simply evolved in-silico.
It's a great product, and there will be people consuming it for sure!
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I think releasing a good, safe engineered product is one of the best way to provide good press to the whole movement.
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Found out: 1700 euros for the jabanese gram positive lux transposon....