T7 expression strain?

[Koeng]

Does anyone a good (preferably cheap) place to get a bacteria strain with the T7 RNA polymerase? I am of the RNA polymerase gene... Thanks!

-Koeng

[DIYBIO Groningen]

I have it.

I have this E.coli strain https://www.neb.com/~/media/Catalog/All-Products/0B28021B9A36470BB46B318DAD19ED4F/Datacards%20or%20Manuals/C2527Datasheet-Lot25.pdf

It has a plasmid coding for the T7 RNA polymerase.

I am not sure I can send you the bacteria but probably I can send you the plasmid that codify for it. We have tu discuss privately about it.

Let me know.

Ale

On 28 Mar 2014, at 02:47, Koeng <koen...@gmail.com> wrote:

Does anyone a good (preferably cheap) place to get a bacteria strain with the T7 RNA polymerase? I am of the RNA polymerase gene... Thanks!

-Koeng

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[Sherman Tang]

Hi Koeng,

If you're having trouble finding a source for the bacteria strain, you may want to explore my company's website as we host research vendors and their service/product offerings. In regards to your request, we have a couple category listings you can consider using to communicate with the vendors listed: Cell Line Construction (https://www.assaydepot.com/service/cell-line-construction) and Strain Development (https://www.assaydepot.com/service/strain-development)

We can also help you source the strain (e.g. we'll communicate with our vendors, generate quotations for you, and provide you a report for you to review for your vendor selection and order). Simply send us an email at exp...@assaydepot.com with your details and our team will work with you right away.

Let me know if you have any questions.

Best regards,

Sherman

[Josiah Zayner]

If you are looking for bacteria that express T7 polymerase you should probably ask around for BL21 cells most any modern lab should have some.

[Koeng]

Yea, it turns out the BL21 cells I had didn't have the DE3 lysogen, (it is so annoying, took me a while to figure out that was the problem.) 

I think one of the other people in my lab have it, thanks!

[Joseph Chemler]

The genotype for many common laboratory strains is given here:
http://openwetware.org/wiki/E._coli_genotypes

great little resource.