DIY Well Water Kit for Coliform Bacteria

[Markos]

Hi,

Would anyone know which reagents are used for this test?

http://www.cleanwaterstore.com/test-kit-bacteria.html#item=L1006690&tab=tab1

http://www.cleanwaterstore.com/blog/diy-well-water-test-kits-for-coliform-bacteria/

Thanks,
Markos

[Nathan McCorkle]

I'd start looking at selective media, MacConkey agar comes to mind first... I'm sure I'll remember some others, but they're zipped up in some neurons that I haven't used in several years.

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[Markos]

Hi Nathan,

"zipped up in some neurons..." :-)

Very good.

Thanks,
Markos

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[Brian Degger]

Hi Markos,

x-glu is the indicator (probably), referenced in 

Handbook of Media for Clinical Microbiology

https://books.google.co.uk/books?id=EiXPAYU9ragC&pg=PA194&lpg=PA194&dq=X-GLU+is+cleaved+by+the+enzyme+%CE%B2-D-Glucosidase,+which+is+characteristic+of+enterococci.+This+results+in+a+color+change+of+the+broth+to+intensive+blue-green&source=bl&ots=i8RoUUrM9N&sig=TPcfhuhHB-UptM8LyFW9H50bMnU&hl=en&sa=X&ved=0ahUKEwjypv2NvvDWAhWmAsAKHXD9Ch0Q6AEIKzAB#v=onepage&q=X-GLU%20is%20cleaved%20by%20the%20enzyme%20%CE%B2-D-Glucosidase%2C%20which%20is%20characteristic%20of%20enterococci.%20This%20results%20in%20a%20color%20change%20of%20the%20broth%20to%20intensive%20blue-green&f=false

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[Markos]

Thank you Brian,

Page 153
The substrate X-glucuronide is used for the identification of β- D -glucuronidase, which is characteristic
for E. coli. E. coli cleaves both Salmon-GAL and X-glucuronide so that positive colonies take on a dark-
blue to violet color.

Page 194
The substrate X-GLU (5-bromo-4-chloro-3-indolyl-β- D -glucopyranoside) is cleaved, stimulated by selected peptones, by the enzyme β- D -glucosidase which is characteristic for enterococci....


Available at:
goo.gl/KdQLdo

or

http://www.bit.ly/2yrlP52

Best Regards,
Markos

-------- Mensagem encaminhada --------

Assunto:	Re: [DIYbio] DIY Well Water Kit for Coliform Bacteria
Data:	Sat, 14 Oct 2017 17:18:43 +0100
De:	Brian Degger <brian....@gmail.com>
Responder a:	diy...@googlegroups.com
Para:	diy...@googlegroups.com <diy...@googlegroups.com>

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