The nanodrop /is/ just a plain old spectrometer, with two fiber optic cables leading to and from the microdrop stage. Search Google for TakeItApart nanodrop to see pics of the inside.
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Excel read/write in python is easy.
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GUIs in python are pretty easy too. Here's some old code I wrote to plot spectrometer data in python:
https://github.com/nmz787/open-spectrometer/blob/master/desktop-software/openSpectrometer.py
I've been working with wxpython lately for GUIs, and would have to look at the pygtk forums and bug tracker to see if it would be worth switching to wxpython or not in any future updates/forks of this. I kind of forgot about it when I was looking at GUI toolkits a few months ago.