Where do you get sequencing done

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Hans Wilms

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Oct 25, 2017, 11:02:26 AM10/25/17
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Hey everyone,
Anyone know of a good place in the US to send a plasmid for a quick Sanger sequencing? A place that will accept a sample sent from my residential address?
--
Hans T. Wilms
PhD student, GSU
Charlie Benson Lab

djwr...@gmail.com

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Oct 25, 2017, 11:36:19 AM10/25/17
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I use Laragen in Culver City, CA


Dan Wright
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Skyler Gordon

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Oct 25, 2017, 5:42:59 PM10/25/17
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Are you looking to sequence a DNA amplicon from the plasmid or are you looking to send the plasmid and have the whole thing sequenced? 

-SG

On Wed, Oct 25, 2017 at 8:36 AM, <djwr...@gmail.com> wrote:
I use Laragen in Culver City, CA


Dan Wright

On Oct 25, 2017, at 8:01 AM, Hans Wilms <hanst...@gmail.com> wrote:

Hey everyone,
Anyone know of a good place in the US to send a plasmid for a quick Sanger sequencing? A place that will accept a sample sent from my residential address?
--
Hans T. Wilms
PhD student, GSU
Charlie Benson Lab

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Learn more at www.diybio.org
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Hans Wilms

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Oct 25, 2017, 5:45:30 PM10/25/17
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Just a DNA amplicon, or just the insert of a plasmid. I was thinking GeneWiz, but just wanted to check if there was anything commonly used by other DIYbiologist.

On Wed, Oct 25, 2017 at 5:42 PM Skyler Gordon <skg...@gmail.com> wrote:
Are you looking to sequence a DNA amplicon from the plasmid or are you looking to send the plasmid and have the whole thing sequenced? 

-SG
On Wed, Oct 25, 2017 at 8:36 AM, <djwr...@gmail.com> wrote:
I use Laragen in Culver City, CA


Dan Wright

On Oct 25, 2017, at 8:01 AM, Hans Wilms <hanst...@gmail.com> wrote:

Hey everyone,
Anyone know of a good place in the US to send a plasmid for a quick Sanger sequencing? A place that will accept a sample sent from my residential address?
--
Hans T. Wilms
PhD student, GSU
Charlie Benson Lab

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Learn more at www.diybio.org
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Skyler Gordon

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Oct 26, 2017, 8:57:51 AM10/26/17
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You want to sequence a DNA amplicon from a plasmid or you want to send them a whole plasmid and have it sequenced?

-SG
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/4F1197F4-DA1F-4E78-B7BE-FAB733FBD143%40gmail.com.

Hans Wilms

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Oct 26, 2017, 2:32:44 PM10/26/17
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Just a DNA amplicon. And thank you, Dan, I'll check it out!

To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/CAGA%2B4LUAd%2BLN5gpq1Xc8ymjQ4qXzmOvmQAcY9P3vfaigrWn0ag%40mail.gmail.com.

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djwr...@gmail.com

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Oct 26, 2017, 2:53:50 PM10/26/17
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Laragen has been cheap and convenient. Doubt if you need a business address since they send nothing to you. You download the sequencing results. 

On Oct 26, 2017, at 11:32 AM, Hans Wilms <hanst...@gmail.com> wrote:

Just a DNA amplicon. And thank you, Dan, I'll check it out!

On Thu, Oct 26, 2017 at 8:57 AM Skyler Gordon <skg...@gmail.com> wrote:
You want to sequence a DNA ng  from a plasmid or you want to send them a whole plasmid and have it sequenced?

Skyler Gordon

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Oct 28, 2017, 8:13:42 PM10/28/17
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Amplicons can be difficult to sequence on new platforms. The massive parallel sequencing isn’t designed to work with populations that have extremely similar sequences.

You may want to see if there is anyone out there providing pyrosequencing services. This method is better / more accurate for determining the sequence of a single gene or amplified product
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