For example, I use λ pl promoter to start the downstream transcription, and I use cI as the upstream signal, or a repressor protein, which would inhibit the transcription of pl promoter.
So I suppose that I should use Hill Kinetics to model this regulation process.
So I need three parameter to describe the regulated transcription rate
1. Hill coefficient or the cooperativity constant n.
In the Wikipedia, it give two conditions with n = 2 and n = 3.
2. Dissociation constant Kd which I don't know
3. The Maximum Transcriptional Rate Vm which I also have no idea of.
Where should I go to find the later two parameters on web?
Is there any database that you always use for such condition?
I am not a professional biology researcher, so I have searched so much but ended up with nothing. Thank you a lot if you can help me!