Free of cost plasmid vector

Tom De Medts

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Sep 22, 2019, 11:10:41 PM9/22/19

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Dear DIYbio community members,

Greetings from the Richmond area, Virginia, USA !

Where / from who can I request, free-of-cost, a small-sized plasmid (<5KBp) that I need to practice / teach the E. coli bacterial transformation protocol ?

I prefer that the plasmid be selected using Kanamycin, because I got a gift of some Kanamycin powder from a retired professor.

Ampicillin selection is also OK I suppose, since I think I should be able to buy some from Amazon, but I prefer not to have that expense.

Thanks, in advance.

- Tom

Dakota Hamill

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Sep 23, 2019, 9:13:55 AM9/23/19

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https://www.carolina.com/biotechnology-plasmid-lambda-dna/puc18-plasmid-10-ug/211462.pr

https://www.carolina.com/biotechnology-plasmid-lambda-dna/pkan-plasmid-40-ug-100-ul-04-ugul/211443.pr?intid=jl_pdp&jl_ctx=on_site

https://www.carolina.com/biotechnology-plasmid-lambda-dna/pgreen-plasmid-1-ug-200-ul-0005-ugul/211449.pr?intid=jl_pdp&jl_ctx=on_site

and many others.

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Nathan McCorkle

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Sep 26, 2019, 4:50:20 PM9/26/19

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the-odin.com has super-cheap plasmids, antibiotics, and media. They're
open to appeals from poor students/beginners too. If you post your
experiments on social media, they also give you store credit.

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Arthur Fernandes

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Oct 10, 2019, 8:05:09 PM10/10/19

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Tom, i can send you a KanR plasmid. Please mail me at fernandes...@gmail.com.

https://www.goldbio.com sells ampicilin quite cheap.

Arthur

AdrianMolecule

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Oct 14, 2019, 1:12:16 PM10/14/19

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Hi Tom,

Odin is quite cheap once you order a larger quantity but they charge you an extra 30$ for delivery which becomes hefty. In here Canada Ottawa where I stated a small DIY lab we did something similar. I could probably send you the transformed bacteria but you'll have to do the plasmid extraction.

Cheers,

Adrian

AdrianMolecule

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Oct 14, 2019, 1:12:16 PM10/14/19

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They are pretty slow to reply or give you credit so don't count on that.

On Thursday, September 26, 2019 at 4:50:20 PM UTC-4, Nathan McCorkle wrote:

the-odin.com has super-cheap plasmids, antibiotics, and media. They're
open to appeals from poor students/beginners too. If you post your
experiments on social media, they also give you store credit.

On Sun, Sep 22, 2019 at 8:10 PM Tom De Medts <tdm...@gmail.com> wrote:
>
> Dear DIYbio community members,
>
> Greetings from the Richmond area, Virginia, USA !
>
> Where / from who can I request, free-of-cost, a small-sized plasmid (<5KBp) that I need to practice / teach the E. coli bacterial transformation protocol ?
>
> I prefer that the plasmid be selected using Kanamycin, because I got a gift of some Kanamycin powder from a retired professor.
>
> Ampicillin selection is also OK I suppose, since I think I should be able to buy some from Amazon, but I prefer not to have that expense.
>
> Thanks, in advance.
> - Tom
>
> --

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> Learn more at www.diybio.org
> ---
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> To unsubscribe from this group and stop receiving emails from it, send an email to diy...@googlegroups.com.

Nathan McCorkle

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Oct 15, 2019, 5:30:40 PM10/15/19

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On Mon, Oct 14, 2019, 10:12 AM AdrianMolecule <buzz...@gmail.com> wrote:

They are pretty slow to reply or give you credit so don't count on that.

My experience has been far from slow. I get my orders in a few days to a week max. Shipping has always been within reason for a given order's weight.

Nathan McCorkle

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Oct 15, 2019, 5:33:18 PM10/15/19

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On Mon, Oct 14, 2019, 10:12 AM AdrianMolecule <buzz...@gmail.com> wrote:

Hi Tom,

Odin is quite cheap once you order a larger quantity but they charge you an extra 30$ for delivery which becomes hefty.

I ordered about $200 worth of products the first time, paid $25 shipping for a huge box full of stuff. Ordered a gel box later, paid $15 shipping. Ordered plasmids later, shipping was free (probably just a promotion).

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