A silly thought regarding plants and yeast

[Sebastian Cocioba]

Hello again guys and gals,

Had a rather silly idea: What if you fuse a plant with a yeast?

How do you think it would best be cultured? What would the resulting cell wall structure/ potential tissue look like?

Hypothetically, one could isolate plant and yeast protoplasts and use PEG to fuse them. Then culture them in some trade-off medium maintaining the osmoticum needed to keep both from popping...assuming that one wont pop in the other's medium. Then embed them in alginate to keep everything together and plate on media with hormones for the plant part and...umm...yeah. Stumped on that front...

Assuming its a true symetric hybrid, what would the resulting cell's needs be? More toward yeast aerobics/anarobics? Sugar would be shared between plant and yeast so thats a given...and it would maintain osmoticum for the most part.

Do you think the CO2 produced by the yeasts fermentation would fuel the photosynthesis and resulting oxygen aid in aerobics in the yeast? Massive assumptions here and I know very little about yeast. Dont feel like "cheating" and looking things up on wiki. I also cant find any literature on this matter at all. Has this ever been done before?

Pardon the mind vomit, just had a thought and wanted to share. :)

[Yuriy Fazylov]

Yes I have thought of that once. Suggested it in Genspace once. Got positive feedback as far as ideas go but never went past that.

Lichen seems to have it good via haustorium. It keeps the components apart yet provides a surface area to share mictonutreants.

Everything after this point is speculative with a hint of goog guess. I would assume something in one would cancel the other. The thing that would really pop your idea out of existance is the shared intron editing mechanism. Fungi do it one way, plants do it the other way.

If you manage to keep the two nuclei apart then you might still have something viable. Yeast can have multiple nuclei if I am not mistaken. Question is, can plants?

A minimal genome yeast with merged with a minimal plant protoplast would be a good start.

I have an idea to keep minimizing a shared genome such as the one you mentioned but not ready to share the though here yet.

After minimizing the genome you could get enough that you could recode, intron replace, and optimize.

[Yuriy Fazylov]

Can't get my hands on this research but your mind vomit might be on to something.
http://link.springer.com/chapter/10.1007/978-94-009-2788-9_89

[Mega [Andreas Stuermer]]

"If you manage to keep the two nuclei apart then you might still have something viable. Yeast can have multiple nuclei if I am not mistaken"

I think that would be the case anyway. What I know, very related plants can fus their nuclei. If you fuse apples with pears you will get separate nuclei.

I imagine Toll-Like Receptors will be a problem. They sense conserved fungal by products in the cell wall, and trigger the innate immune system to fight it. Remember, plants have no adaptive immune system, so their innate IS will be pretty well developed.

[Aspen Young]

I stumbled upon this: http://biochem80p.wordpress.com/tag/example-of-multi-nuclei-cells/

[Alexey Zaytsev]

http://libgen.org/search.php?req=4c4e48f33a16f6bfbc256e6babc7e673&open=0&view=simple&column=md5 if interested.

But, 1987? Does not looks very fresh.

On Thu, Aug 28, 2014 at 9:17 AM, Yuriy Fazylov <yuriy...@gmail.com> wrote:

Can't get my hands on this research but your mind vomit might be on to something.
http://link.springer.com/chapter/10.1007/978-94-009-2788-9_89

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[Yuriy]

I can imagine a system in which the threshold for toxicity Is dialed down.
https://en.m.wikipedia.org/wiki/Karyoklepty