15$ PCR machine working properly

[Subin e k]

I Have been designing a PCR machine for a month ago. Now the designs complete and have completed the analysis.The machine is working properly but the intensity of amplified bands are too low.The machine was cross tested with a Bio Rad machine in my department laboratory and was analysed in UV it gives some unwanted amplification also.My theory was right i was actually able to make the machine work.Now going for the secondary design having a peltier element and a new mechanism for faster cooling and heating

"The results of my Machine is attached as a picture with it"........."Please see it and give your suggestions"

Subin

Dept Of Biotechnology

National institute of technology Calicut

[jarlemag]

That's cool. To give some suggestions though, I'd think you need to share some details about the design. Do you have a picture of the machine itself?

Best regards,

JP

[Subin e k]

The picture of the prototype i have made

[jarlemag]

Congratulations on getting it working, I bet you can improve your results a lot with some refinements. How is the machine controlled? And how did you make the heatblock?

Best regards,

JP

[Claudio Tiecher]

congratulations!

I would also like a bit more details about the design... maybe a picture of the inside :)

cheers,
Claudio