Backup of the whole individual genome

[Mike Petersen]

Hi everybody,

let´s say I wanted to "backup" me whole genome, let it be for scientific reasons or for medical purposes in future.
What options are available at the moment to get a digital backup for your whole individual genome (not only some SNPs or the exome)
Thank you

Mike

[Mega [Andreas Stuermer]]

Safest way is get some of your tissue or blood, extract tons of DNA, put it on filter paper. Dry it. Stable DNA has a very long half-live (500 years IIRC) at room temperature. Put it into your freezer, so it should survive the next million years (assuming your freezer will be functional that long).

In 10 years you probably can sequence it for a few bucks. 

[Mega [Andreas Stuermer]]

Dried DNA*

[Koeng]

You could try to sequence your entire genome. Usually this is a pretty difficult task, although most people here probably have better advice on specifically doing that. If you do your own library prep, at best it'd be around 900

After that, just store it on a flashdrive. If you just want to store your fasta genome sequence, it should only take a few gbs. 

-Koeng

[Cathal (Phone)]

For longterm storage, store on a suitable RAID array rather than a single flashdrive, or use some error correcting software to make the sequence data on the flashdrive super redundant. Check manufacturer's warranties on device lifetime if kept in archival conditions. Better yet, find a techie librarian and ask them how they store large archival data.

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[Bryan Bishop]

On Mon, Mar 16, 2015 at 10:07 AM, Koeng <koen...@gmail.com> wrote:

You could try to sequence your entire genome.

You may miss out on methylation depending on which procedures you use. I would go for physical storage and sequencing at your convenience.

- Bryan
http://heybryan.org/
1 512 203 0507

[Cathal (Phone)]

Truth here! DNA is highly context sensitive. OTOH, methylation patterns will also vary highly between tissues and tissue lineages, so if you want a really good idea of your genetic and developmental "background" maybe you should store bone marrow, an extracted vein (got any varicose?) and some epithelial tissues from externalities and brainstem.

Ideally, freeze yourself in carbonite. ;)

[Koeng]

I'll agree, this is true. Store it for later, it'll be much cheaper to sequence in the future. If you have a reason that you need the sequence now, do it now, but if you just want it *just in case* store it

[Mike Petersen]

So now is the question: what is the right way to store your DNA for a later sequencing.
Keep it in my own freezer? Or do you know any commercial companies that would store it for me?
I think with 23andme it´s an option, that they keep your cells and store it and not only the digital data.

[Mega [Andreas Stuermer]]

1,2and me don't sequence everything, they just look for SNPs right? So you will have mutations they are not looking for thus rhey won't find it. Or do they offer whole-genome?
Methylation?

Best way is to get a genomic DNA purification kit, and then put it on filter paper. Dry and package wrapped in plastic foil. So temperature doesnt matter much (keep it from light though. Plus the colder the better)

[Gavin Scott]

Honestly if you're doing it for the benefit of yourself, why not just
keep the DNA where it is now, and deal with the issues when you have a
use for the information? Sequencing is just going to get better/more
complete/cheaper, and it's very safely stored right now, (and when it
isn't any more then you probably don't care so much about it :)

Do you have a goal/purpose in mind? It makes a big difference as to
whether you want multiple tissues/epigenetics vs simple genomic
sequencing.

G.

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[Dakota Hamill]

For as long as you're alive you'll have a backup of your personal genome.  Once it's sequenced, just store it as a notepad file.

To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/CA%2BcsFZj7co56Mh5m8su01_CrqeNoGW4uwSsA97z8P8CmaxXYQw%40mail.gmail.com.

[SC]

If you're looking for your genome data to live on after you're gone, submit it to Genbank.

For sequencing, check out Illumina or PacBio's new chemistry.  23andme just checks a set of loci, they won't provide complete sequencing.

[Steve]

You could choose to go for the ultimate long term storage:
http://www.extremetech.com/extreme/199414-scientists-create-million-year-data-storage-with-dna
... though the cost and effort required does smack of overkill (and infinite recursion) -> DNA extracted, whole genome (and epigenome) sequenced, digitized and reencoded for storage as DNA then encapsulated in silica spheres :)

[Cathal Garvey]

No expense can be spared in preserving my genes, unless you can think of
an easier way to replicate my DNA outside my own corpus!

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[Jonathan Cline]

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On Monday, March 16, 2015 at 7:57:45 AM UTC-7, Mike Petersen wrote: