Cheapest polymerase?

[Koeng]

Hey

I have been shopping for some inexpensive polymerase (high fidelity (pfu and such) and taq), and wanted to know if anyone here knows of an ultra-cheap supplier. Thanks

-Koeng

[Koeng]

http://www.aasinc.co/store-and-promotions.html#!/EasyPfu-DNA-Polymerase/p/52930720/category=13912399

this looks pretty good

[Koeng]

http://www.ebay.com/itm/Taq-DNA-Polymerase-UltraPure-Grade-HPLC-purified-1-000-units-/301250297676?hash=item4623eabf4c:g:ZOkAAOSwQItTzDq7

and what I found for taq. I haven't used either one of these options. 

-Koeng

[Dakota Hamill]

I'd rather pay an extra $20 and get Taq from NEB vs some rando off ebay.  But show me the gels and I might be a believer.

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[Sebastian S Cocioba]

Just ran some rubisco large subunit primers on a bunch of plants I isolated earlier today. I got a 5mL master mix from Gene and Cell Technologies on eBay. Works a treat. Never an issue. Its just taq not pfu but I've even done larger amplicon pcr and sequence came back spotless. The empty or missing bands were from samples with too little or no DNA present. Qubit 2.0 confirmed the claim. 

Sebastian S. Cocioba

CEO & Founder

New York Botanics, LLC

Blog: ATinyGreenCell.com

To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/CAGdeWmSGu0ECerxFiHcjTwWz_OwfxKWRVaOt5Jajah%2B22PD1hw%40mail.gmail.com.