transient GFP plant

[Mega [Andreas Stuermer]]

Hi guys, 

I took some pics of the fluorescent plant I created in the Ars Electronica. 

Google doesn't let me post the pictures, so I gotta outsource. http://diyspartanbiotech.wordpress.com/2014/02/12/better-pics-of-the-transient-gfp-plant-in-soil/ and http://diyspartanbiotech.wordpress.com/2014/02/05/transient-gfp-transformation-agroinfiltration-worked/

The GFP in a Ti plasmid was kindly donated by T.Moravec, Institute of Expertimental Botany, Prague. 

Did this as a waste of time until my pGlowroplast comes from synthesis/+PCR ligation. What the heck takes them so long? :/ Well, I wanted to save money so they're PCRing the lux genes instead of synthesizing and they're having trouble with the PCR... 

[Mega [Andreas Stuermer]]

Btw, if anyone happens to be in Linz in the next time, I'd happily show the plants :D 

[Cathal Garvey]

Awesome, Andreas! Congratulations!

On 12/02/14 22:17, Mega [Andreas Stuermer] wrote:
> Btw, if anyone happens to be in Linz in the next time, I'd happily show the
> plants :D
>

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[Mega [Andreas Stuermer]]

Thanks!

Actually it wasn't very difficult at all, and I would rather have worked on my real glowing plants. But I figured I had to do an experiment where I'd get quick results to be able to show to my friends^^

[Nathan McCorkle]

It would be nice to show your non-transformed control plants under
attempted fluorescence... a bit hard to compare to the
Professor-provided images. Upon closer inspection it does appear that
your plants are glowing but are very spotted in appearance
comparatively. Can you elaborate on why this might be? Did you dip the
whole plant into Agro solution, or did you spray on Agro with a
water-sprayer?

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[Mega [Andreas Stuermer]]

Of course I also have pics of untransformed plants. That means, I didn't make such with my cell phone camera. ^^ 

Well, they are just red (weak chlorophyll autofluorescence). 

I transformed them only transiently, i.e. I pipetted 100 uL of agrobacterium solution onto a leave and streaked it with the tip. Only the spots where agro touched the plants show fluorescence. 

[Cathal Garvey]

Try illuminating Pesto someday. When you decouple photosynthesis (by
mashing up plant bits, basically), there's no place for all that UV to
go, and chlorophyll's orange/red autofluorescence gets really strong! :)

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[Mega [Andreas Stuermer]]

Something like GFP pesto? :D

[Cathal Garvey]

I'd buy it!

On 14/02/14 23:49, Mega [Andreas Stuermer] wrote:
> Something like GFP pesto? :D

>

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[Nathan McCorkle]

On Fri, Feb 14, 2014 at 3:49 PM, Mega [Andreas Stuermer]
<masters...@gmail.com> wrote:
> Something like GFP pesto? :D
>

Maybe this would work better for the luminescent system that needed
oxygen? I think there was one discussed that was known to produce the
fuel and the enzymes, but that needed lots of oxygen to power and that
was diffusion limited... or maybe I'm thinking of the GFP beer/bread
posts where people have said oxygen is needed to get GFP to properly
fold.

[Andreas Stuermer]

Yeah, it may well be one of the main limiting faxctors. I guess we'll find out some day ;) 

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