Bacteria which can't grow at 33C?

[Antony Evans]

Hello,

I'm looking for a bacteria strain which is amenable to transformation and can't grow at 33 degrees Celsius (so that it can't grow in warm blooded animals).

Any suggestions?

Antony

[Yuriy]

keyword it: Psychrophile

[Antony Evans]

Thanks, that's actually too cold for us. Should have specified we also want them to be able to grow at room temperature.

i'm starting to explore marine bacteria on the insight that corals die when temperatures get too high.

Antony

[Yuriy]

Eh.
why don't you look into a coral whose temperature range doesn't exceed that specified? ~25-33 +\- 2 deg., C?
Look if it has endo-bacteria is amenable to transformation.

This way if you can create a bacteria that doesn't wash out, and has circuits and kill switch for both itself and thus to corals. Marketing opportunity ;)

It "can't grow at 33 degrees Celsius (so that it can't grow in warm blooded animals.) we ... want them to be able to grow at room temperature."

"corals die when temperatures get too high."

What do warm blooded animals have to do with it?

Go to a well experienced pet-shop. Ask them why they have chillers for their marine and frag tanks.

[Koeng]

Possibly program a temperature sensitive lambda red system with a positive feedback system loop connected to a toxin. There are varieties which repress at 30 but not at higher temperatures, like 37. Don't know if you could get as specific as 33c.

-Koeng

[Matt Lawes]

How about something like Streptomyces or Myxobacteria? They do well around 28-30C but really hate mid / high 30s.

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[Dakota Hamill]

Streptomyces chop off massive pieces of their DNA at random and shuffle things constantly.  Transforming them is terrible.  Plasmid loss is common.  Integrated DNA loss is common.

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