Will gloves from sandbox cabinet fit to the biosafety class III box?

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Dorif

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Apr 1, 2017, 6:05:39 AM4/1/17
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I've got some parts from old class III biosafety box and want to re-construct it (why not?), so I wonder if sandbox gloves can be used there. Gloves like this: http://www.ebay.com/itm/Sandblasting-protective-gloves-emperor-Mediumweight-ME-107-Industrial-gloves-/152394504337?var=&hash=item237b6b9891:m:mbynRJWZQmDjluj1c3CycVw or http://www.ebay.com/itm/Sandblasting-protective-gloves-60cm-Latex-industrial-glove-blast-cabinet-/351983756267?var=&hash=item51f3dde3eb:m:mrvVoIoxGY5w-M627MJFQqg or http://www.ebay.com/itm/1-PAIRE-GLOVE-SANDBLASTING-PRO-LAT460-/132126635600?var=&hash=item1ec35c7650:m:mWDZu8_5U_wmZPQA25uRXZA

Of course, I won't work there with something BSL 4, but it can make biologists life a lot of easier - no need to worry about contamination from air when seeding cells, bacteria or preparing PCR mix, dNTPs or primers.

And will it be effective without air circulation at all (just closed box: if you want to put something in - UV before, something out - UV after, if not UV sensivive, or UV sensitive parts are protected - UV while in box)? Air hood is heavily damaged (well, demolished...) and needs too much effort.

Thanks a lot!

MC

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Apr 4, 2017, 12:06:40 PM4/4/17
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I'm curious too, after see the price of actual replacement glove box gloves. I've also considered using arm-length cleaning gloves (similar to dishwashing gloves).

Dennis Oleksyuk

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Apr 4, 2017, 4:14:15 PM4/4/17
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If the only purpose of using the gloves is to protect the media from contamination from outside, then I bet that any gloves will do much better than open air.  

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John Griessen

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Apr 11, 2017, 5:00:11 PM4/11/17
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On 04/01/2017 05:05 AM, Dorif wrote:
> And will it be effective without air circulation at all (just closed box? Air hood is heavily damaged


For making a small "clean-room" work area, it is the flow that isolates things, so moving air is necessary. When air is moving in
a designed flow bench,
1. the dirt particles are pulled to the filter,
2. the moving fan is behind/on-the-dirty-side-of the filter and baffles prevent turbulence from reaching the filter surface,
3. filtered air exits the work area with low turbulence,
4. returning most of the air exiting the work area to be pulled through again is better than drawing in only new room air
if the room is ordinary cleanliness,
5. pressure inside the work area is positive relative to the room.

Because of the above causes, the effect is a small tendency of particles lodged on filter to come loose, and
a large tendency of floating particles to become lodged on filter -- if not the first pass through, then the next passes.
vibration and bumping shocks to the cabinet need to be avoided for clean-room-like results.

So any kind of redesign needs to have the above statements be true/positive for every piece of the design. With no fan or flow,
only 3. would be true and the rest false...

Nathan McCorkle

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Apr 11, 2017, 10:12:10 PM4/11/17
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On Sat, Apr 1, 2017 at 3:05 AM, Dorif <dor...@gmail.com> wrote:
> And will it be effective without air circulation at all (just closed box:

I use a plastic storage tub/bin, with holes cut in the side to make a
semi-closed box for subculturing... it is not fun or enjoyable to use,
but it gives me a lot of peace-of-mind over working on my home
benchtop. I definitely would prefer to work in a clean-air hood... but
I don't have the floor space to accomodate this, since I am not doing
sterile work too often. If you are ready to get into this hobby
full-swing, definitely look into buying or building a flow-hood.
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