Somatropin

[Aaron Chapman]

Can someone point me in the direction on how to synthesize Somatropin? Or at least where to start researching?

Sent from my iPhone

[Dakota Hamill]

HGH is another expensive biologic, and probably a grey area drug, since it's so often associated with steroid use.  

Things like HGH can save someones life, or destroy it.  Hormones are pretty powerful and their chemistry is quite interesting, especially since the timing of their release and the dosages have such a profound affect upon the body and mind.

That said, google would give you everything you need to know.  Wikipedia will probably even give you the amino acid sequence, and i'm sure the DNA sequence for the protein is published.    I don't think you'll be able to express it in E. Coli and inject it to get huge though.

On Sun, Jan 25, 2015 at 12:06 AM, Aaron Chapman <rednec...@gmail.com> wrote:

Can someone point me in the direction on how to synthesize Somatropin? Or at least where to start researching?

Sent from my iPhone

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[Aaron Chapman]

Thank you for your response. I will come back with better questions after i research it more.

[Cathal Garvey]

For high quality amino sequence don't trust Wikipedia, go directly to
uniprot.org. Prefer gold-starred sequences, and feel inspired to read
the references, if any. Get to know your protein; does it need
post-translational modification? Is there a signal peptide that needs
cleaving? Does it get glycosylated? Does it need help folding? Are there
cysteine for disulphide bridges?

On 26/01/15 04:16, Dakota Hamill wrote:
> HGH is another expensive biologic, and probably a grey area drug, since
> it's so often associated with steroid use.
>
> Things like HGH can save someones life, or destroy it. Hormones are
> pretty powerful and their chemistry is quite interesting, especially
> since the timing of their release and the dosages have such a profound
> affect upon the body and mind.
>
> That said, google would give you everything you need to know. Wikipedia
> will probably even give you the amino acid sequence, and i'm sure the
> DNA sequence for the protein is published. I don't think you'll be
> able to express it in E. Coli and inject it to get huge though.
>
>
>
>
>
> On Sun, Jan 25, 2015 at 12:06 AM, Aaron Chapman <rednec...@gmail.com
> <mailto:rednec...@gmail.com>> wrote:
>
> Can someone point me in the direction on how to synthesize
> Somatropin? Or at least where to start researching?
>
> Sent from my iPhone
>
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> -- You received this message because you are subscribed to the
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> Learn more at www.diybio.org <http://www.diybio.org>

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>

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[Nathan McCorkle]

On Mon, Jan 26, 2015 at 12:06 AM, Cathal Garvey
<cathal...@cathalgarvey.me> wrote:
> For high quality amino sequence don't trust Wikipedia, go directly to
> uniprot.org. Prefer gold-starred sequences, and feel inspired to read the
> references, if any. Get to know your protein; does it need
> post-translational modification? Is there a signal peptide that needs
> cleaving? Does it get glycosylated? Does it need help folding? Are there
> cysteine for disulphide bridges?

If DIYbio had a homepage with useful information for beginners, I
think this should
be the answer to 'how do I synthesize X (which is a protein)'.

[Mega [Andreas Stuermer]]

If you are asking how to get started to express proteins, try this

http://diyspartanbiotech.files.wordpress.com/2014/03/genetic-engineering-and-synbio-for-beginners-v1.pdf